Retinal axons in homozygous third instar larvae show frequent projection errors, including crossovers (52 +/- 4%) and bundling defects (61 +/- 9%). The lamina plexus is disrupted by large gaps (47 +/- 5%).

Segmental nerve axons in homozygous embryos have notably thickened longitudinal bundles and they leave the central nervous system at altered projection angles compared to wild type. In 57% of animals, the spacing of the neuromeres is narrowed, with the distance between the anterior commissural and posterior commissural axon bundles of adjacent neuromeres being shortened. There are large breaks in the longitudinal axon bundles as assayed by Fas2 staining, and these breaks coincide with the thickening of the longitudinal bundles in each neuromere.