FlyBase Allele Report: Dmel\Tsc1[29]

General Information

Symbol

Dmel\Tsc1²⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0123968

Feature type

allele

Associated gene

Dmel\Tsc1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Nucleotide substitution: C?T.

(Gao and Pan, 2001)

Amino acid replacement: Q61term.

(Gao and Pan, 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:24,130,906..24,130,906 [+]

Nucleotide change:

C24130906T

Reported nucleotide change:

C?T|FBrf0136941

Amino acid change:

Q61term | Tsc1-PA

Reported amino acid change:

Q61term

Comment:

Site of nucleic acid difference inferred by FlyBase based on reported amino acid change

(Gao and Pan, 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Tsc1²⁹ somatic cyst stem clones in the testis are progressively lost.

(Yuen et al., 2021)

Young Tsc1²⁹ mosaic flies (<2 days old) have all seven rhabdomeres, while almost no rhabdomeres are present in the eyes of Tsc1²⁹ mosaic flies after 20 hours of light/dark cycling.

(Wang et al., 2009)

Tsc1²⁹ mitotic cell cycle defective clones show an increase in TR-avidin uptake.

(Hennig et al., 2006)

100% of Tsc1²⁹ homozygotes die as second instar larvae.

(Zhang et al., 2003)

Tsc1²⁹ homozygotes die as first instar larvae, soon after hatching. In somatic clones of Tsc1²⁹ in the eye, cell size is increased by a factor of 1.9 compared to their wild-type neighbours.

(Gao et al., 2002)

Homozygous somatic clones in the eye leads to ommatidia that are larger than wild type. Rhabdomeres are approximately 1.8 times the size of wild-type counterparts in area. However, the organisation of ommatidia and the differentiation of various cell types are nearly normal, with occasional loss of photoreceptor cells. Eye discs that are more than 90% mutant for Tsc1 are 2.9 times the size of wild-type discs in area. Clones in third instar larval imaginal discs have larger nuclei and an increased cell size. Also clones have (up to over 3 times) more cells than their wild-type twin spots. Nucleolus area in clones is also twice the size of neighbouring wild-type cells, but there is no increase in DNA content in these clones - the cells are diploid. Homozygous mutant clones in the wing margin leads to larger wing margin bristles.

(Gao and Pan, 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Tsc1²⁹ has increased cell size | somatic clone phenotype, enhanceable by Hsc70-4^e3

(Hennig et al., 2006)

Suppressed by

Statement

Reference

Tsc1²⁹ has decreased cell number | somatic clone | spermatogenesis phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/bam^UASp.GFP

(Yuen et al., 2021)

Tsc1²⁹ has lethal | larval stage phenotype, suppressible by Rheb^PΔ1/Rheb[+]

(Zhang et al., 2003)

Tsc1²⁹ has increased cell size | somatic clone phenotype, suppressible by mTor^2L1/Tor[+]

(Gao et al., 2002)

Tsc1²⁹ has lethal | recessive | larval stage phenotype, suppressible by mTor^2L1/Tor[+]

(Gao et al., 2002)

Enhancer of

Statement

Reference

Tsc1²⁹ is an enhancer of increased cell size | somatic clone phenotype of Hsc70-4^e3

(Hennig et al., 2006)

Tsc1²⁹/Tsc1[+] is an enhancer of increased cell death phenotype of mTor^ey.Tag:FLAG

(Hennig et al., 2006)

NOT Enhancer of

Statement

Reference

Tsc1²⁹/Tsc1[+] is a non-enhancer of partially lethal - majority die phenotype of hpo^+t4.0, hpo^42-20

(Wu et al., 2003)

Suppressor of

Statement

Reference

Tsc1²⁹ is a suppressor | partially of abnormal neuroanatomy | somatic clone phenotype of Hr51^LL04325

(Yaniv et al., 2012)

Tsc1²⁹, Tsc1[+], gig[+], gig¹⁹² is a suppressor | partially of female sterile phenotype of path^KG06640

(Goberdhan et al., 2005)

Tsc1²⁹, Tsc1[+], gig[+], gig¹⁹² is a suppressor | partially of decreased fecundity | female phenotype of path^KG06640

(Goberdhan et al., 2005)

Tsc1²⁹, Tsc1[+], gig[+], gig¹⁹² is a suppressor of decreased body size phenotype of path^KG06640

(Goberdhan et al., 2005)

Tsc1²⁹, Tsc1[+], gig[+], gig¹⁹² is a suppressor of decreased cell number phenotype of path^KG06640

(Goberdhan et al., 2005)

Tsc1²⁹, Tsc1[+], gig[+], gig¹⁹² is a suppressor of decreased cell size phenotype of path^KG06640

(Goberdhan et al., 2005)

Tsc1²⁹, Tsc1[+], gig[+], gig¹⁹² is a suppressor of abnormal developmental rate phenotype of path^KG06640

(Goberdhan et al., 2005)

Tsc1²⁹ is a suppressor of decreased cell size | somatic clone phenotype of Akt¹

(Gao and Pan, 2001)

Tsc1²⁹ is a suppressor of lethal | recessive | larval stage phenotype of InR³⁵

(Gao and Pan, 2001)

Tsc1²⁹ is a suppressor of decreased cell size | somatic clone phenotype of InR³⁵

(Gao and Pan, 2001)

Tsc1²⁹ is a suppressor of lethal phenotype of InR⁰⁵⁵⁴⁵/InR³⁵³

(Gao and Pan, 2001)

NOT Suppressor of

Statement

Reference

Tsc1²⁹/Tsc1[+] is a non-suppressor of partially lethal - majority die phenotype of hpo^+t4.0, hpo^42-20

(Wu et al., 2003)

Tsc1²⁹/Tsc1²⁹ is a non-suppressor of decreased cell size | somatic clone phenotype of mTor^2L1

(Gao et al., 2002)

Other

Statement

Reference

Tsc1²⁹, gig[+]/gig¹⁹² has increased body size phenotype

(Goberdhan et al., 2005)

Rheb^PΔ1, Tsc1²⁹ has melanotic mass phenotype phenotype

(Zhang et al., 2003)

Rheb^PΔ1/Rheb[+], Tsc1²⁹ has melanotic mass phenotype phenotype

(Zhang et al., 2003)

InR³⁵, Tsc1²⁹ has partially lethal - majority live phenotype

(Gao and Pan, 2001)

InR⁰⁵⁵⁴⁵/InR³⁵³, Tsc1²⁹ has partially lethal - majority live phenotype

(Gao and Pan, 2001)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Tsc1²⁹ has wing margin bristle | somatic clone phenotype, non-enhanceable by gig¹⁹²

(Gao and Pan, 2001)

Suppressed by

Statement

Reference

Tsc1²⁹ has cyst progenitor cell | somatic clone phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/bam^UASp.GFP

(Yuen et al., 2021)

Tsc1²⁹ has cyst cell of testis | somatic clone phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/bam^UASp.GFP

(Yuen et al., 2021)

NOT suppressed by

Statement

Reference

Tsc1²⁹ has wing margin bristle | somatic clone phenotype, non-suppressible by gig¹⁹²

(Gao and Pan, 2001)

Enhancer of

Statement

Reference

Tsc1²⁹/Tsc1[+] is an enhancer of eye phenotype of mTor^ey.Tag:FLAG

(Hennig et al., 2006)

NOT Enhancer of

Statement

Reference

Tsc1²⁹ is a non-enhancer of wing margin bristle | somatic clone phenotype of gig¹⁹²

(Gao and Pan, 2001)

Suppressor of

Statement

Reference

Tsc1²⁹ is a suppressor | partially of gamma Kenyon cell | somatic clone phenotype of Hr51^LL04325

(Yaniv et al., 2012)

Tsc1²⁹, Tsc1[+], gig[+], gig¹⁹² is a suppressor of wing phenotype of path^KG06640

(Goberdhan et al., 2005)

NOT Suppressor of

Statement

Reference

Tsc1²⁹ is a non-suppressor of wing margin bristle | somatic clone phenotype of gig¹⁹²

(Gao and Pan, 2001)

Other

Statement

Reference

Hsc70-4^e3, Tsc1²⁹ has retina phenotype

(Hennig et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The developmental regrowth defect seen in Hr51^LL04325 MARCM neuroblast clones is partially suppressed in clones that are doubly mutant for Hr51^LL04325 and Tsc1²⁹.

(Yaniv et al., 2012)

Tsc1²⁹ gig¹⁹² double mutants carrying both Tsc1^S533A.Tub and gig^4A.αTub are viable and normal in size, although they show a slight reduction in lipid levels compared to wild type.

(Schleich and Teleman, 2009)

Tsc1²⁹ does not protect Df(1)su(s)R194/+ clones in the eye; Df(1)su(s)R194/+ ; Tsc1²⁹ clones are not recovered in the adult eye in animals with mosaic eyes containing two genotypes of cells with respect to RpL36; cells which are Df(1)su(s)R194/+ and cells in which the haplo-insufficiency of Df(1)su(s)R194/+ for RpL36 has been rescued by RpL36^+t4 (in a wild-type background the Df(1)su(s)R194/+ clones are eliminated by cell competition and are not seen in the adult eye in these animals). Also, Tsc1²⁹ does not prevent apoptosis of Df(1)su(s)R194/+ cells in the wing.

(Tyler et al., 2007)

The severity of the Tor^{ey.hs.T:Zzzz\FLAG} phenotype is strongly enhanced by a heterozygous Tsc1²⁹ background.

Imaginal disc cells double mutant for Hsc70-4^e3 and Tsc1²⁹ exhibit a synergistic increase in cell size as compared with their single mutant counterparts. There is an exacerbation of Tsc1²⁹-induced tissue overgrowth in the presence of the Hsc70-4^e3 mutation, in some cases resulting in a marked outgrowth of tissue in the anterior portion of the retina.

(Hennig et al., 2006)

Tsc1²⁹/+, gig¹⁹²/+ double mutants are slightly larger than wild-type flies.

The path^KG06640 growth and developmentally delayed phenotypes are significantly rescued by the presence of combined heterozygous Tsc1²⁹ and gig¹⁹² mutations. About 10% of rescued females are able to lay a small number of eggs, some of which can develop into adult flies.

(Goberdhan et al., 2005)

The second instar larval lethality of Rheb^PΔ1 homozygotes is partially suppressed by Rheb^PΔ1/+: 61% survive to third instar and 21% pupate. These larvae often contain melanotic 'tumors'.

(Zhang et al., 2003)

The first instar larval lethality due to Tsc1²⁹ is partially suppressed by heterozygosity for Tor^2L1 : 31% (n = 193) of Tor^2L1/+ Tsc1²⁹/Tsc1²⁹ animals survive to the pupal stage. The increased cell size seen in somatic clones of Tsc1²⁹ in the eye is partially suppressed in a Tor^2L1 heterozygous background: the resulting clone cells are 1.3X the size of those outside the clone, compared to 1.9X for Tsc1²⁹ somatic clone cells in a wild-type background. Cells in Tor^2L1; Tsc1²⁹ double mutant somatic clones are approximately 0.25 times the size of their heterozygous neighbours - i.e.- have little or no size difference with Tor^2L1 somatic clone cells.

(Gao et al., 2002)

Clones that are homozygous for both gig¹⁹² and Tsc1²⁹ show an identical phenotype to homozygous clones of either gig¹⁹² or Tsc1²⁹ alone. Pten^dj189, Tsc1²⁹ double mutant somatic clones show an additive cell size increase phenotype. Tsc1²⁹ suppresses the lethality of homozygous InR³⁵ or InR³⁵³/InR⁰⁵⁵⁴⁵, allowing 6.6% and 39% animals to survive to adults respectively.

(Gao and Pan, 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Tsc1²⁹ is rescued by Tsc1^Tub.WT

(Schleich and Teleman, 2009)

Tsc1²⁹ is rescued by Tsc1^S533A.Tub

(Schleich and Teleman, 2009)

Tsc1²⁹ is rescued by Tsc1^S533D.Tub

(Schleich and Teleman, 2009)

Tsc1²⁹ is rescued by Tsc1^+t4.7

(Gao and Pan, 2001)

Comments

Tsc1²⁹ flies carrying either Tsc1^Tub.WT, Tsc1^S533A.Tub or Tsc1^S533D.Tub are viable and normal in size.

(Schleich and Teleman, 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

TSC1²⁹

(Tyler et al., 2007)

Tsc1²⁹

(Yuen et al., 2021, Yaniv et al., 2012, Zheng and Sehgal, 2010, Chang and Neufeld, 2009, Wang et al., 2009, Hennig et al., 2006, Goberdhan et al., 2005)

dtsc1²⁹

(Zhang et al., 2014)

tsc1²⁹

(Vachias et al., 2025, Li et al., 2019, Schleich and Teleman, 2009)

Name Synonyms

Secondary FlyBase IDs

References (17)

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