FlyBase Allele Report: Dmel\lqf[FDD9]

General Information

Symbol

Dmel\lqf^FDD9

Species

D. melanogaster

Name

FlyBase ID

FBal0104483

Feature type

allele

Associated gene

Dmel\lqf

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Cadavid et al., 2000)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G?A. The nucleotide substitution is in intron 6 which generates a cryptic splice acceptor site, which results in a C-terminally truncated mRNA.

(Overstreet et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:7,535,530..7,535,530 [+]

Nucleotide change:

G7535530A

Reported nucleotide change:

G?A

Comment:

This mutation creates a cryptic splice acceptor which adds 12 bases from the intron onto the subsequent exon.

(Overstreet et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable

(Xie et al., 2012, Cadavid et al., 2000)

visible

(Cho and Fischer, 2011)

visible | recessive | heat sensitive

(Cadavid et al., 2000)

Phenotype Manifest In

eye | heat sensitive

(Cadavid et al., 2000)

eye photoreceptor cell

(Cho and Fischer, 2011)

eye photoreceptor cell & ommatidium | ectopic

(Overstreet et al., 2004)

eye photoreceptor cell | cell non-autonomous | ectopic | somatic clone

(Cadavid et al., 2000)

leg sensillum | ectopic | heat sensitive

(Cadavid et al., 2000)

mystery cell | heat sensitive

(Cadavid et al., 2000)

ommatidium

(Cho and Fischer, 2011)

ommatidium | heat sensitive

(Cadavid et al., 2000)

photoreceptor neuron | ectopic | heat sensitive

(Cadavid et al., 2000)

tarsal segment | heat sensitive

(Cadavid et al., 2000)

wing | heat sensitive

(Cadavid et al., 2000)

wing margin bristle | heat sensitive

(Cadavid et al., 2000)

wing vein | heat sensitive

(Cadavid et al., 2000)

Detailed Description

Statement

Reference

Homozygous eyes are rough and many ommatidia contain an abnormal number of photoreceptor cells.

(Cho and Fischer, 2011)

The ommatidia of lqf^FDD9 homozygous adults have extra photoreceptors.

(Overstreet et al., 2004)

Homozygotes are nearly wild-type at 18^oC but have eye and wing defects at 25^oC. At 25^oC, the eyes contain facets with ectopic photoreceptor cells. The additional photoreceptor cells arise from the 'mystery cells' which are present early during eye development. Wings have thickenings of the longitudinal veins and clumps of bristles and notching at the wing margin. Legs have fused and shortened tarsal segments. Second and third legs have defects similar to those in the first legs and a variety of bristle duplications are also seen. lqf^FDD9/lqf^S011027 flies have similar but more severe phenotypes than those of lqf^FDD9 homozygotes. When homozygous lqf^FDD9 clones are induced in the eye, phenotypically mutant facets at the clone border containing ectopic photoreceptors are seen in which each photoreceptor, including the ectopic one, is lqf⁺.

(Cadavid et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

lqf^FDD9 has visible | recessive phenotype, enhanceable by Chc[+]/Chc⁴

(Cadavid et al., 2000)

lqf^FDD9 has visible | recessive phenotype, enhanceable by AP-2α³/alpha-Adaptin[+]

(Cadavid et al., 2000)

lqf^FDD9 has visible | recessive phenotype, enhanceable by shi¹

(Cadavid et al., 2000)

lqf^FDD9 has visible | recessive phenotype, enhanceable by shi²

(Cadavid et al., 2000)

Suppressed by

Statement

Reference

lqf^FDD9 has visible phenotype, suppressible by Rala[+]/Rala^EE1

(Cho and Fischer, 2011)

lqf^FDD9 has visible phenotype, suppressible by Rala[+]/Rala^PG69

(Cho and Fischer, 2011)

Other

Statement

Reference

lqf^FDD9, neur[+]/neur¹ has lethal phenotype

(Overstreet et al., 2004)

lqf^FDD9, neur¹ has lethal phenotype

(Overstreet et al., 2004)

Chc¹, lqf^FDD9 has lethal phenotype

(Cadavid et al., 2000)

Chc[+]/Chc¹, lqf^FDD9 has lethal phenotype

(Cadavid et al., 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

lqf^FDD9 has eye photoreceptor cell & ommatidium | ectopic phenotype, enhanceable by neur[+]/neur¹

(Overstreet et al., 2004)

lqf^FDD9 has eye phenotype, enhanceable by Chc[+]/Chc⁴

(Cadavid et al., 2000)

lqf^FDD9 has eye phenotype, enhanceable by AP-2α³/alpha-Adaptin[+]

(Cadavid et al., 2000)

lqf^FDD9 has wing phenotype, enhanceable by AP-2α³/alpha-Adaptin[+]

(Cadavid et al., 2000)

NOT Enhanced by

Statement

Reference

lqf^FDD9 has phenotype, non-enhanceable by spen^L13

(Eun et al., 2007)

lqf^FDD9 has phenotype, non-enhanceable by spen^14O1

(Eun et al., 2007)

lqf^FDD9 has phenotype, non-enhanceable by spen^16H1

(Eun et al., 2007)

lqf^FDD9 has phenotype, non-enhanceable by aux⁷²⁷

(Eun et al., 2007)

lqf^FDD9 has phenotype, non-enhanceable by aux^D136

(Eun et al., 2007)

lqf^FDD9 has phenotype, non-enhanceable by aux^C2

(Eun et al., 2007)

lqf^FDD9 has phenotype, non-enhanceable by aux^N7

(Eun et al., 2007)

lqf^FDD9 has phenotype, non-enhanceable by aux^D128

(Eun et al., 2007)

lqf^FDD9 has phenotype, non-enhanceable by aux^L7

(Eun et al., 2007)

lqf^FDD9 has phenotype, non-enhanceable by aux^F37

(Eun et al., 2007)

lqf^FDD9 has phenotype, non-enhanceable by spen^C18

(Eun et al., 2007)

lqf^FDD9 has phenotype, non-enhanceable by spen^J47

(Eun et al., 2007)

Suppressed by

Statement

Reference

lqf^FDD9 has ommatidium phenotype, suppressible by Rala[+]/Rala^EE1

(Cho and Fischer, 2011)

lqf^FDD9 has eye photoreceptor cell phenotype, suppressible by Rala[+]/Rala^EE1

(Cho and Fischer, 2011)

lqf^FDD9 has ommatidium phenotype, suppressible by Rala[+]/Rala^PG69

(Cho and Fischer, 2011)

lqf^FDD9 has eye photoreceptor cell phenotype, suppressible by Rala[+]/Rala^PG69

(Cho and Fischer, 2011)

lqf^FDD9 has eye phenotype, suppressible by Hsap\EPN2^ΔENTH.ro

(Overstreet et al., 2003)

lqf^FDD9 has eye phenotype, suppressible by Hsap\EPN2^ro.PO

(Overstreet et al., 2003)

lqf^FDD9 has eye phenotype, suppressible by Rnor\Epn1^ΔENTH.ro

(Overstreet et al., 2003)

lqf^FDD9 has eye phenotype, suppressible by Rnor\Epn1^ro.PO

(Overstreet et al., 2003)

Other

Statement

Reference

Chc[+]/Chc¹, lqf^FDD9 has tarsal segment phenotype

(Cadavid et al., 2000)

Chc[+]/Chc¹, lqf^FDD9 has wing phenotype

(Cadavid et al., 2000)

Chc[+]/Chc¹, lqf^FDD9 has eye phenotype

(Cadavid et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Rala^EE1 and Rala^PG69 each dominantly suppress the rough eye phenotype of lqf^FDD9 homozygotes, with a greater proportion of ommatidia containing the normal number of photoreceptor cells in the double mutant flies.

(Cho and Fischer, 2011)

Unlike lqf^FDD9/lqf^FDD9 animals, lqf^FDD9/lqf^FDD9; neur¹/+ die as larvae. The number of photoreceptors per ommatidium in lqf^FDD9/lqf^FDD9 flies is increased still further by neur¹¹/+.

(Overstreet et al., 2004)

The eye defects of lqf^FDD9 homozygotes are dominantly enhanced by Chc⁴. The Chc¹/+ ; lqf^FDD9/lqf^FDD9 combination is lethal. Rare escapers have severely malformed eyes and tarsal fusions and the wing phenotype is enhanced compared to lqf^FDD9 homozygotes. The eye and wing defects of lqf^FDD9 homozygotes are dominantly enhanced by α-Adaptin³. The lqf^FDD9 phenotype is enhanced if the flies are also homozygous for shi¹ or shi².

(Cadavid et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

lqf^FDD9 is rescued by lqf^FL.GFP

(Xie et al., 2012)

lqf^FDD9 is rescued by lqf^ΔENTH.GFP

(Xie et al., 2012)

lqf^FDD9 is rescued by lqf^ΔCBM.GFP

(Xie et al., 2012)

lqf^FDD9 is rescued by lqf^ΔDPW.GFP

(Xie et al., 2012)

lqf^FDD9 is rescued by lqf^ΔNPF.GFP

(Xie et al., 2012)

lqf^FDD9 is rescued by lqf^ΔUIM2.GFP

(Xie et al., 2012)

lqf^FDD9 is rescued by lqf^UIM1.EA.GFP

(Xie et al., 2012)

lqf^FDD9 is rescued by lqf^{ΔCBM.ΔDPW1.GFP}

(Xie et al., 2012)

lqf^FDD9 is rescued by lqf^{ΔCBM.ΔDPW.GFP}

(Xie et al., 2012)

lqf^FDD9 is rescued by lqf^4xNPF.GFP

(Xie et al., 2012)

lqf^FDD9 is rescued by lqf^ENTH.RA.GFP

(Xie et al., 2012)

lqf^FDD9 is rescued by lqf^ENTH.TD.GFP

(Xie et al., 2012)

lqf^FDD9 is rescued by lqf^{ENTH.FTVF.RDAA.GFP}

(Xie et al., 2012)

lqf^FDD9 is rescued by lqf^ro.Tag:FLAG

(Overstreet et al., 2003)

lqf^FDD9 is rescued by lqf^{ΔDPW.ro.Tag:FLAG}

(Overstreet et al., 2003)

lqf^FDD9 is rescued by lqf^{ΔEN2.ro.Tag:FLAG}

(Overstreet et al., 2003)

lqf^FDD9 is rescued by lqf^{ΔCBM.ro.Tag:FLAG}

(Overstreet et al., 2003)

lqf^FDD9 is rescued by lqf^+t19G

(Cadavid et al., 2000)

Partially rescued by

lqf^FDD9 is partially rescued by lqf^ΔUIM.GFP

(Xie et al., 2012)

lqf^FDD9 is partially rescued by lqf^{UIM1.EA.ΔUIM2.GFP}

(Xie et al., 2012)

lqf^FDD9 is partially rescued by lqf^{ENTH.RWRK.AAAA.GFP}

(Xie et al., 2012)

lqf^FDD9 is partially rescued by lqf^{ΔEN1.ro.Tag:FLAG}

(Overstreet et al., 2003)

lqf^FDD9 is partially rescued by lqf^{ΔENTH.ro.Tag:FLAG}

(Overstreet et al., 2003)

lqf^FDD9 is partially rescued by lqf^{ENTH.ro.Tag:FLAG}

(Overstreet et al., 2003)

lqf^FDD9 is partially rescued by lqf^ro.Tag:FLAG

(Cadavid et al., 2000)

Not rescued by

lqf^FDD9 is not rescued by lqf^ENTH.UIM.GFP

(Xie et al., 2012)

Comments

lqf^{ΔENTH.T:Avic\GFP} completely rescues the morphological defects of lqf^FDD9 flies.

lqf^{ΔUIM.T:Avic\GFP} only slightly rescues the morphological defects of lqf^FDD9 flies, with 70 +/- 4% of facets in the eye appearing wild type.

lqf^{ENTH.RWRK.AAAA.T:Avic\GFP} only slightly rescues the morphological defects of lqf^FDD9 flies, with 16 +/- 5% of facets in the eye appearing wild type.

(Xie et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

25667

w^*; st¹ lqf^FDD9/TM6B, Tb¹

Notes on Origin

Discoverer

Comments

Allelic series based on viability: lqf^FDD9 < lqf^bE248 < lqf^S011027 < lqf^BT < lqf^ARI = lqf^L895 = lqf^L71.

(Overstreet et al., 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

lqf^FDD9

(Xie et al., 2012, Cho and Fischer, 2011, Eun et al., 2008, Eun et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (7)

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