FlyBase Allele Report: Dmel\shot[unspecified]

General Information

Symbol

Dmel\shot^unspecified

Species

D. melanogaster

Name

FlyBase ID

FBal0062790

Feature type

allele

Associated gene

Dmel\shot

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

FlyBase curator comment: this entry is used to capture phenotypic information when the particular allele (or allele combination) used by the author could not be determined but the context of the experiment suggests that the phenotype being described is some kind of loss of function.

(FlyBase Curators, 2025-)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Alves-Silva et al., 2012)

lethal - all die before end of larval stage | recessive

(Prout et al., 1997)

some die during larval stage | recessive

(Prout et al., 1997)

visible | somatic clone

(Prout et al., 1997)

Phenotype Manifest In

dendrite & motor neuron | embryonic stage

(Bottenberg et al., 2009)

embryonic/larval motor neuron | embryonic stage

(Alves-Silva et al., 2012)

embryonic proventriculus

(Fuss et al., 2004)

larva

(Prout et al., 1997)

proventriculus inner layer primordium

(Fuss et al., 2004)

proventriculus intermediate layer primordium

(Fuss et al., 2004)

tendon cell

(Alves-Silva et al., 2012)

tendon cell | embryonic stage 17

(Bottenberg et al., 2009)

wing | somatic clone

(Prout et al., 1997)

Detailed Description

Statement

Reference

Motorneurons are shorter in mutant embryos compared to wild type. Tendon cells are disrupted in mutant embryos.

(Alves-Silva et al., 2012)

Dendrites of aCC and RP2 motorneurons in shot^unspecified mutant embryos display severely reduced complexity in morphology at late embryonic stages compared to controls. In contrast, at the onset of dendrite formation (embryonic stage 15 = 12 hours), there are no obvious aberrations in shot^unspecified mutant animals compared to wild-type.

In late stage embryos, shot^unspecified mutant tendon cells are dramatically elongated, held together only through their persisting apico-basal F-actin arrays.

(Bottenberg et al., 2009)

In shot^unspecified mutants, initial formation of the ectodermal keyhole region is normal, but the inward movement of these cells into the endodermal keyhole domain fails to occur. Furthermore, the rim of the proventriculus is significantly reduced in size.

(Fuss et al., 2004)

Mutants show no gross defects in anterior-posterior or dorsal-ventral patterning.

(Gao et al., 1999)

Homozygotes die as early larvae. Homozygous clones in the wing produce discrete, round blisters of variable size. These blisters can be located anywhere on the wing. Wing venation is normal. Homozygous embryos show no gross morphological abnormalities at hatching. Hatched larvae fail to grow.

(Prout et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

shot^unspecified/shot[+] is an enhancer of visible | recessive phenotype of mys⁸

(Prout et al., 1997)

Other

Statement

Reference

Eb1⁰⁴⁵²⁴, shot^unspecified/shot[+] has abnormal neuroanatomy phenotype

(Alves-Silva et al., 2012)

Phenotype Manifest In

Enhancer of

Statement

Reference

shot^unspecified/shot[+] is an enhancer of wing phenotype of mys⁸

(Prout et al., 1997)

Other

Statement

Reference

Eb1⁰⁴⁵²⁴, shot^unspecified/shot[+] has axon phenotype

(Alves-Silva et al., 2012)

Eb1⁰⁴⁵²⁴, shot^unspecified/shot[+] has microtubule phenotype

(Alves-Silva et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

shot^unspecified/+ ; Eb1⁰⁴⁵²⁴/+ double heterozygous embryonic neurons in primary culture show shortening of axons and increase disorganisation of the microtubules compared to either single heterozygote.

(Alves-Silva et al., 2012)

Shows a weak genetic interaction with mys⁸; the frequency of wing blisters is increased from approximately 10% in mys⁸ single hemizygotes to approximately 20% in mys⁸ hemizygotes that are also heterozygous for shot^unspecified. Shows no interaction with mys^b9.

(Prout et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

shot^unspecified is rescued by Scer\GAL4^sr-md710/shot^LA.UAS.GFP

(Alves-Silva et al., 2012)

shot^unspecified is rescued by Scer\GAL4^sr-md710/shot^{RE-3MtLSmut.UAS.GFP}

(Alves-Silva et al., 2012)

shot^unspecified is rescued by Scer\GAL4^unspecified/shot^LA.UAS.GFP

(Bottenberg et al., 2009)

shot^unspecified is rescued by Scer\GAL4^sr-md710/shot^LA.UAS.GFP

(Bottenberg et al., 2009)

shot^unspecified is rescued by Scer\GAL4^sr-md710/shot^{LA-ΔEF-hand.UAS.GFP}

(Bottenberg et al., 2009)

Partially rescued by

shot^unspecified is partially rescued by shot^LA.UAS.GFP/Scer\GAL4^eve.RN2O

(Alves-Silva et al., 2012)

shot^unspecified is partially rescued by Scer\GAL4^sr-md710/shot^LC.UAS.GFP

(Bottenberg et al., 2009)

shot^unspecified is partially rescued by shot^{LA-ΔPlakin.UAS.GFP}/Scer\GAL4^unspecified

(Bottenberg et al., 2009)

shot^unspecified is partially rescued by shot^{LA-ΔPlakin.UAS.GFP}/Scer\GAL4^Vsx1-MzVum

(Bottenberg et al., 2009)

shot^unspecified is partially rescued by Scer\GAL4^unspecified/shot^{LA-Δrod1.UAS.GFP}

(Bottenberg et al., 2009)

shot^unspecified is partially rescued by Scer\GAL4^sr-md710/shot^{LA-Δrod1.UAS.GFP}

(Bottenberg et al., 2009)

Not rescued by

shot^unspecified is not rescued by shot^{RE-ΔCtail.UAS.GFP}/Scer\GAL4^eve.RN2O

(Alves-Silva et al., 2012)

shot^unspecified is not rescued by Scer\GAL4^sr-md710/shot^{RE-ΔCtail.UAS.GFP}

(Alves-Silva et al., 2012)

shot^unspecified is not rescued by shot^{RE-3MtLSmut.UAS.GFP}/Scer\GAL4^eve.RN2O

(Alves-Silva et al., 2012)

shot^unspecified is not rescued by Scer\GAL4^unspecified/shot^LC.UAS.GFP

(Bottenberg et al., 2009)

shot^unspecified is not rescued by Scer\GAL4^unspecified/shot^{LA-ΔEF-hand.UAS.GFP}

(Bottenberg et al., 2009)

shot^unspecified is not rescued by shot^{LA-ΔGAS2.UAS.GFP}/Scer\GAL4^unspecified

(Bottenberg et al., 2009)

shot^unspecified is not rescued by Scer\GAL4^sr-md710/shot^{LA-ΔGAS2.UAS.GFP}

(Bottenberg et al., 2009)

Comments

Expression of shot^{LA.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^eve.RN2O significantly rescues the motor neuron stall phenotype seen in shot^unspecified embryos in vivo.

Expression of either shot^{RE-ΔCtail.Scer\UAS.T:Avic\GFP} or shot^{RE-3MtLSmut.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^eve.RN2O does not rescue the motor neuron stall phenotype seen in shot^unspecified embryos in vivo.

Expression of either shot^{LA.Scer\UAS.T:Avic\GFP} or shot^{RE-3MtLSmut.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^sr-md710 restores tendon cell integrity in shot^unspecified embryos.

Expression of shot^{RE-ΔCtail.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^sr-md710 fails to rescue tendon cell integrity in shot^unspecified embryos.

(Alves-Silva et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

shot^-

(Fuss et al., 2004)

shot^unspecified

unspecified

(Prout et al., 1997)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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