FlyBase Allele Report: Dmel\msn[172]

General Information

Symbol

Dmel\msn¹⁷²

Species

D. melanogaster

Name

FlyBase ID

FBal0061020

Feature type

allele

Associated gene

Dmel\msn

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

X ray

Nature of the Allele

Allele class

Mutagen

X ray

(Su et al., 1998, Treisman et al., 1997)

Progenitor genotype

Caused by aberration

In(3)msn¹⁷²

Cytology

Description

Inversion breakpoint within the coding region.

(Su et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates carcinoma

modeled by Ras85D^V12.UAS

(Kong et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible

(Treisman et al., 1997)

Phenotype Manifest In

actin filament | somatic clone

(Horne-Badovinac et al., 2012)

egg | germline clone

(Treisman et al., 1997)

egg | somatic clone

(Horne-Badovinac et al., 2012)

egg chamber | somatic clone

(Horne-Badovinac et al., 2012)

embryo | dorsal closure stage

(Koppen et al., 2006)

embryonic/first instar larval cuticle | dorsal

(Su et al., 1998)

embryonic segment | dorsal closure stage

(Koppen et al., 2006)

eye photoreceptor cell

(Treisman et al., 1997)

eye photoreceptor cell | somatic clone

(Paricio et al., 1999)

filamentous actin | dorsal closure stage

(Koppen et al., 2006)

follicle cell | somatic clone

(Horne-Badovinac et al., 2012)

ommatidial precursor cluster | somatic clone

(Paricio et al., 1999)

ommatidium | somatic clone

(Paricio et al., 1999)

photoreceptor cell R4 | somatic clone

(Paricio et al., 1999)

rhabdomere

(Treisman et al., 1997)

rhabdomere | somatic clone

(Paricio et al., 1999)

Detailed Description

Statement

Reference

Females carrying homozygous follicle cell clones can produce round eggs.

The planar organisation of basal actin filaments in the follicle cell epithelium at oogenesis stage 7 is disrupted in when the follicle epithelium is primarily composed of homozygous cells, such that the filaments no longer align perpendicular to the anterior-posterior axis. Stage 10 egg chambers containing homozygous follicle cell clones are often shorter and rounder than normal.

(Horne-Badovinac et al., 2012)

2% of msn¹⁷² mutant embryos display an anterior dorsal hole, 38% complete dorsal closure but show misalignment of segments and 12% show embryonic lethality without obvious defects during dorsal closure. msn¹⁷² embryos show intermittent breaks in the actin distribution at the epithelial margin, typically affecting groups of 2-4 cells. Affected cells are wider and less elongated than neighboring cells with normal actin localization. 30% of the embryos display segment misalignments during the final phase of dorsal closure. The segment misalignment defect is typically accompanied by unusual irregularities in the width of segments during the closure process. Some segments appear abnormally wide, while neighboring segments are excessively constricted.

(Koppen et al., 2006)

In msn¹⁷² somatic clones in the eye ommatidia often have malformed or missing photoreceptors. Those ommatidia that have the normal complement of photoreceptors are often misrotated and display the wrong chiral form or are symmetrical. In mosaic photoreceptor R3/R4 pairs, the msn⁺ photoreceptor has a strong preference for adopting the R3 fate. This can often lead to chirality inversions where the msn⁺ R4 precursor adopts the R3 position displacing the msn¹⁷² R3 precursor. The mosaic analysis also showed that for correct 90^o rotation there is no bias for any particular photoreceptor.

(Paricio et al., 1999)

Homozygous or msn¹⁰²/msn¹⁷² embryos show defects in dorsal closure.

(Su et al., 1998)

Clones in the eye are externally rough. They contain elongated rhabdomeres and some ommatidia also contain missing or an extra photoreceptor. Eggs from germline clones are never laid.

(Treisman et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

msn¹⁷²/msn¹⁷² is an enhancer of neoplasia | larval stage | somatic clone - tissue specific phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI

(Kong et al., 2021)

msn¹⁷²/msn[+] is an enhancer of partially lethal - majority die phenotype of slpr^BS06

(Polaski et al., 2006)

NOT Enhancer of

Statement

Reference

msn¹⁷²/msn[+] is a non-enhancer of visible phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

Suppressor of

Statement

Reference

msn¹⁷²/msn[+] is a suppressor | partially of visible phenotype of Rab11^mo

(Tiwari and Roy, 2009)

msn¹⁷²/msn[+] is a suppressor | partially of visible phenotype of Rab11^N124I.UAS, Scer\GAL4^GMR.PF

(Tiwari and Roy, 2009)

msn¹⁷²/msn[+] is a suppressor | partially of visible phenotype of Rala^35d

(Balakireva et al., 2006)

msn¹⁷²/msn[+] is a suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF, egr^UAS.cMa

(Moreno et al., 2002)

msn¹⁷² is a suppressor | partially of abnormal planar polarity phenotype of Scer\GAL4^hs.2sev, Tak1^UAS.cMa

(Mihaly et al., 2001)

msn¹⁷²/msn[+] is a suppressor of abnormal planar polarity phenotype of dsh^hs.sev.B

(Paricio et al., 1999)

NOT Suppressor of

Statement

Reference

msn¹⁷²/msn[+] is a non-suppressor of visible phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

Other

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, msn¹⁷² has some die during larval stage phenotype

(Kong et al., 2021)

Phenotype Manifest In

Suppressed by

Statement

Reference

msn¹⁷² has embryonic/first instar larval cuticle | dorsal phenotype, suppressible by Jra^Asp.hs.sev

(Su et al., 1998)

msn¹⁷²/msn¹⁰² has embryonic/first instar larval cuticle | dorsal phenotype, suppressible by tkv^{Q253D.UAS.cNb}/Scer\GAL4^69B

(Su et al., 1998)

Enhancer of

Statement

Reference

msn¹⁷²/msn¹⁷² is an enhancer of eye disc | somatic clone - tissue specific phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI

(Kong et al., 2021)

NOT Enhancer of

Statement

Reference

msn¹⁷²/msn[+] is a non-enhancer of nurse cell ring canal phenotype of Scer\GAL4^{VP16.mat.αTub67C}, dock^{UASp.Tag:HA,Tag:Myr(Unk)}

(Stark et al., 2021)

msn¹⁷², msn[+], WASp[+], WASp¹ is a non-enhancer of nurse cell ring canal phenotype of Scer\GAL4^{VP16.mat.αTub67C}, dock^{UASp.Tag:HA,Tag:Myr(Unk)}

(Stark et al., 2021)

msn¹⁷²/msn[+] is a non-enhancer of wing hair phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

Suppressor of

Statement

Reference

msn¹⁷²/msn[+] is a suppressor | partially of eye phenotype of Rab11^N124I.UAS, Scer\GAL4^GMR.PF

(Tiwari and Roy, 2009)

msn¹⁷²/msn[+] is a suppressor | partially of eye phenotype of Rab11^mo

(Tiwari and Roy, 2009)

msn¹⁷²/msn[+] is a suppressor | partially of microchaeta phenotype of Rala^35d

(Balakireva et al., 2006)

msn¹⁷²/msn[+] is a suppressor of macrochaeta phenotype of Rala^35d

(Balakireva et al., 2006)

msn¹⁷² is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PF, egr^GS9830

(Igaki et al., 2002)

msn¹⁷²/msn[+] is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, egr^UAS.cMa

(Moreno et al., 2002)

msn¹⁷² is a suppressor | partially of eye phenotype of Scer\GAL4^hs.2sev, Tak1^UAS.cMa

(Mihaly et al., 2001)

msn¹⁷² is a suppressor | partially of eye photoreceptor cell phenotype of Scer\GAL4^hs.2sev, Tak1^UAS.cMa

(Mihaly et al., 2001)

msn¹⁷²/msn[+] is a suppressor of ommatidium phenotype of dsh^hs.sev.B

(Paricio et al., 1999)

NOT Suppressor of

Statement

Reference

msn¹⁷²/msn[+] is a non-suppressor of nurse cell ring canal phenotype of Scer\GAL4^{VP16.mat.αTub67C}, dock^{UASp.Tag:HA,Tag:Myr(Unk)}

(Stark et al., 2021)

msn¹⁷², msn[+], WASp[+], WASp¹ is a non-suppressor of nurse cell ring canal phenotype of Scer\GAL4^{VP16.mat.αTub67C}, dock^{UASp.Tag:HA,Tag:Myr(Unk)}

(Stark et al., 2021)

msn¹⁷²/msn[+] is a non-suppressor of wing hair phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The eye defects seen in Rab11^mo homozygotes are partially suppressed by msn¹⁷²/+.

The reduced eye size caused by expression of Rab11^{N124I.Scer\UAS} under the control of Scer\GAL4^GMR.PF is partially suppressed by msn¹⁷²/+.

(Tiwari and Roy, 2009)

msn¹⁷², suppresses the ommatidial polarity defects seen in dsh^hs.sev.B flies grown at 29^oC. About 80% of ommatidia are in their correct polarity compared to 55% in dsh^hs.sev.B alone.

(Paricio et al., 1999)

The dorsal open phenotype of msn¹⁰²/msn¹⁷² embryos is partially rescued by Jra^Asp.hs.sev expressed using heat shock or by tkv^{Q253D.Scer\UAS.cNb} expressed under the control of Scer\GAL4^69B.

(Su et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

msn¹⁷² is rescued by msn^UAS.cSa/Scer\GAL4^69B

(Su et al., 2000)

Partially rescued by

msn¹⁷² is partially rescued by msn^-CT.UAS/Scer\GAL4^69B

(Su et al., 2000)

Not rescued by

msn¹⁷² is not rescued by Scer\GAL4^69B/msn^KD.UAS

(Su et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

5947

w^*; msn¹⁷² P{neoFRT}80B/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

msn¹⁷²

(Marcogliese et al., 2022, Kiely and Gilbert-Ross, 2021, Kong et al., 2021, Stark et al., 2021, Horne-Badovinac et al., 2012, Cobreros-Reguera et al., 2010, Djiane and Mlodzik, 2010, Tiwari and Roy, 2009, Balakireva et al., 2006, Delaney et al., 2006, Koppen et al., 2006, Polaski et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (19)

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