FlyBase Allele Report: Dmel\pb[UAS.A]

General Information

Symbol

Dmel\pb^UAS.A

Species

D. melanogaster

Name

FlyBase ID

FBal0059978

Feature type

allele

Associated gene

Dmel\pb

Associated Insertion(s)

Carried in Construct

P{UAS-pb.A}

Also Known As

UAS-pb

Key Links

Transgenic product class

cDNA

(Aplin and Kaufman, 1997)

wild_type

(Aplin and Kaufman, 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Aplin and Kaufman, 1997)

wild_type

(Aplin and Kaufman, 1997)

Mutagen

in vitro construct

(Aplin and Kaufman, 1997)

Progenitor genotype

Carried in construct

P{UAS-pb.A}

Cytology

Description

UASt regulatory sequences drive expression of a full-length pb cDNA.

(Aplin and Kaufman, 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^69B

(Aplin and Kaufman, 1997)

lethal, with Scer\GAL4^71B

(Aplin and Kaufman, 1997)

lethal - all die before end of pupal stage, with Scer\GAL4^dpp.blk1

(Aplin and Kaufman, 1997)

some die during pupal stage, with Scer\GAL4^dpp.blk1

(Aplin and Kaufman, 1997)

Phenotype Manifest In

antenna, with Scer\GAL4^dpp.blk1

(Aplin and Kaufman, 1997)

crossvein, with Scer\GAL4^dpp.blk1

(Aplin and Kaufman, 1997)

embryonic tritocerebrum, with Scer\GAL4^sca-537.4

(Sprecher et al., 2004)

eye, with Scer\GAL4^dpp.blk1

(Benassayag et al., 2003)

eye, with Scer\GAL4^ey.PH

(Benassayag et al., 2003)

imaginal disc, with Scer\GAL4^dpp.blk1

(Abzhanov et al., 2001)

leg, with Scer\GAL4^30A

(Aplin and Kaufman, 1997)

leg, with Scer\GAL4^dpp.blk1

(Benassayag et al., 2003, Aplin and Kaufman, 1997)

macrochaeta, with Scer\GAL4^dpp.blk1

(Aplin and Kaufman, 1997)

tarsal segment, with Scer\GAL4^30A

(Aplin and Kaufman, 1997)

tarsal segment, with Scer\GAL4^dpp.blk1

(Aplin and Kaufman, 1997)

wing, with Scer\GAL4^dpp.blk1

(Benassayag et al., 2003, Aplin and Kaufman, 1997)

wing & macrochaeta, with Scer\GAL4^dpp.blk1

(Aplin and Kaufman, 1997)

wing margin, with Scer\GAL4^sd-SG29.1

(Adachi-Yamada et al., 2005)

wing vein, with Scer\GAL4^dpp.blk1

(Aplin and Kaufman, 1997)

wing vein, with Scer\GAL4^sd-SG29.1

(Adachi-Yamada et al., 2005)

Detailed Description

Statement

Reference

When pb^Scer\UAS.A is driven by Scer\GAL4^sd-SG29.1 abnormal wing vein patterns and incisions in the wing margin are seen.

(Adachi-Yamada et al., 2005)

Expression of pb^Scer\UAS.A under the control of Scer\GAL4^sca-537.4 results in a mutant phenotype in the embryonic tritocerebrum. The phenotype has a penetrance of more than 70-80%.

(Sprecher et al., 2004)

When pb^Scer\UAS.A is driven by Scer\GAL4^dpp.blk1 a substantial eye loss phenotype as well as wing and leg defects are seen. When pb^Scer\UAS.A is driven by Scer\GAL4^ey.PH a substantial or full eye loss phenotype is seen. When driven by Scer\GAL4^GMR.PF no effect is seen.

(Benassayag et al., 2003)

Most imaginal discs expressing pb^Scer\UAS.A under the control of Scer\GAL4^dpp.blk1 are misshapen; the discs have abnormal folding and do not lay flat like wild-type discs.

(Abzhanov et al., 2001)

Expression of pb^Scer\UAS.A under the control of Scer\GAL4^lab.PH does not result in morphological defects in the tritocerebrum or any other part of the embryonic brain.

(Hirth et al., 2001)

Scer\GAL4^dpp.blk1-mediated expression in the imaginal discs results in striking growth and patterning defects in the antenna, legs and wings. Upon eclosion flies are unable to pull themselves free of the pupal case. Labial-like structures are observed along the T1 legs, maxillary palp-like structures are formed along the lateral aspects of the legs in some locations. General leg patterning defects include a shortening and distortion of the leg along the PD axis, fusion of tarsal segments and large bulbous outgrowths of tissue along the lateral surfaces. Wings do not exhibit homeotic transformation but are smaller in size than wild type, are missing both longitudinal and cross veins, have ectopic socketed bristles growing from the wing blade surface and display a crumpled appearance. Ectopic expression does not increase apoptosis in the wing, leg, haltere or eye discs. Scer\GAL4^69B- or Scer\GAL4^71B-mediated expression causes embryonic or early larval lethality. Scer\GAL4^30A-mediated expression causes minor non-homeotic disruptions of the legs that include fusion of tarsal segments.

(Aplin and Kaufman, 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

pb^UAS.A/Scer\GAL4^lz-gal4 is a suppressor of visible phenotype of Scer\GAL4^lz-gal4, sens^UAS.cNa

(Anderson et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

pb^UAS.A/Scer\GAL4^lz-gal4 is a suppressor of eye phenotype of Scer\GAL4^lz-gal4, sens^UAS.cNa

(Anderson et al., 2011)

pb^UAS.A/Scer\GAL4^pb.PJ is a suppressor of pseudotrachea phenotype of Scer\GAL4^pb.PJ, ci^UAS.cAa

(Joulia et al., 2005)

pb^UAS.A/Scer\GAL4^dpp.blk1 is a suppressor of eye | ectopic phenotype of Scer\GAL4^dpp.blk1, ey^UAS.cHa

(Benassayag et al., 2003)

pb^UAS.A/Scer\GAL4^lab.PH is a suppressor of embryonic tritocerebrum phenotype of lab¹⁴

(Hirth et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of pb^Scer\UAS.A strongly suppresses the disorganised eye phenotype caused by expression of sens^Scer\UAS.cNa under the control of Scer\GAL4^lz-gal4.

(Anderson et al., 2011)

When pb^Scer\UAS.A is overexpressed along with ci^Scer\UAS.cAa, both under the control of Scer\GAL4^pb.PJ, the ci^Scer\UAS.cAa phenotype is suppressed and labial palps appear almost the same as wild type.

(Joulia et al., 2005)

Expression of pb^Scer\UAS.A under the control of Scer\GAL4^lab.PH rescues the tritocerebral defects seen in lab¹⁴ embryonic brains. 41.7% of embryos show a complete rescue of the defects (taking into account that the phenotypic penetrance of the lab¹⁴ phenotype is 88.6%).

(Hirth et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

7298

w¹¹¹⁸; P{UAS-pb.A}49.1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

pb^Scer\UAS.A

pb^UAS.A

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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