FlyBase Allele Report: Dmel\Raf[E1]

General Information

Symbol

Dmel\Raf^E1

Species

D. melanogaster

Name

FlyBase ID

FBal0051293

Feature type

allele

Associated gene

Dmel\Raf

Associated Insertion(s)

Carried in Construct

Also Known As

D-raf^E1

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Hata et al., 1994)

Progenitor genotype

Cytology

Description

Amino acid replacement: P637S.

(Hata et al., 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:2,342,991..2,342,991 [+]

Nucleotide change:

C2342991T

Amino acid change:

P594S | Raf-PA; P594S | Raf-PE

Reported amino acid change:

P637S

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Lu et al., 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eclosion | heat sensitive

(Hata et al., 1994)

decreased occurrence of cell division | heat sensitive

(Hata et al., 1994)

lethal | heat sensitive

(Hata et al., 1994)

Phenotype Manifest In

imaginal disc | heat sensitive

(Hata et al., 1994)

Detailed Description

Statement

Reference

Temperature shift experiments demonstrate the latest temperature-sensitive period for the requirement of phl during development is at the late pupal stage. Downshift experiment shows that embryos never hatch even when they were transferred to 17^oC at 3 hours AEL at 28^oC. The temperature-sensitive period for maternal phl function is 60 minutes AEL at 28^oC and 260 minutes AEL at 17^oC. Temperature shift up to 28^oC within three days after puparium formation causes a failure to develop to mature adult within the pupal case, a raise in temperature after this period allows development to mature adult but individuals exhibit an eclosion defect. Mitotic clones demonstrate a proliferation defect, imaginal discs exhibit growth retardation. At 28^oC the cell cycle is arrested at a phase or phases other than the M-phase.

(Hata et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

phl[+]/Raf^E1 is a suppressor of visible phenotype of Dref^UAS.cSa, Scer\GAL4^en-e16E

(Yoshida et al., 2004)

Phenotype Manifest In

Suppressor of

Statement

Reference

phl[+]/Raf^E1 is a suppressor of wing vein L5 | distal phenotype of Dref^UAS.cSa, Scer\GAL4^en-e16E

(Yoshida et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

phl^E1/+ significantly suppresses the extra vein formation seen in flies expressing Dref^Scer\UAS.cSa under the control of Scer\GAL4^en-e16E.

(Yoshida et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

D-raf^E1

(Yoshida et al., 2004, Hata et al., 1994)

Raf^E1

phl^E1

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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