FlyBase Allele Report: Dmel\robo1[1]

(Evans and Bashaw, 2010, Spitzweck et al., 2010, Kim et al., 2002, Fritz and VanBerkum, 2000, Rajagopalan et al., 2000, Sun et al., 2000, Battye et al., 1999)

larval longitudinal connective (with robo1⁸)

(Spitzweck et al., 2010)

larval longitudinal connective (with robo1^BG01092)

(Berni et al., 2008)

larval longitudinal connective | embryonic stage 16

(Hsouna et al., 2003)

larval MP1 neuron

(Fritz and VanBerkum, 2000)

larval multidendritic class IV neuron

(Dimitrova et al., 2008)

larval multidendritic class IV neuron | somatic clone

(Dimitrova et al., 2008)

larval neuron

(Garbe et al., 2007, Kidd et al., 1998)

larval segmental nerve sensory neuron

(Parsons et al., 2003)

larval thoracic lateral multidendritic neuron ldaA

(Parsons et al., 2003)

larval ventral nerve cord

(Rajagopalan et al., 2000)

larval ventral nerve cord | embryonic stage

(Reichert et al., 2016, Brown et al., 2015)

larval ventral nerve cord commissure | embryonic stage

(Garbe et al., 2007)

lesA neuron

(Parsons et al., 2003)

medial longitudinal fascicle

(Evans and Bashaw, 2012)

motor neuron (with robo1²)

(Mauss et al., 2009)

motor neuron | somatic clone

(Brierley et al., 2009)

muscle cell of A1-7 lateral oblique muscle 1

(Kramer et al., 2001)

muscle cell of A1-7 ventral longitudinal muscle 3

(Kramer et al., 2001)

muscle cell of A1-7 ventral longitudinal muscle 4

(Kramer et al., 2001)

neuroblast | increased number | somatic clone

(de Torres-Jurado et al., 2022)

neuropil | somatic clone

(Brierley et al., 2009)

pCC neuron

(Hiramoto and Hiromi, 2006, Fritz and VanBerkum, 2002, Fritz and VanBerkum, 2000)

pCC neuron | embryonic stage

(Brown et al., 2015)

presumptive embryonic/larval central nervous system (with robo1^BG01092)

(Berni et al., 2008)

presumptive embryonic salivary gland

(Kolesnikov and Beckendorf, 2005)

symmetrical commissure

(Simionato et al., 2007)

symmetrical commissure (with robo1^BG01092)

(Berni et al., 2008)

symmetrical commissure | embryonic stage

(Ratnaparkhi et al., 2002)

tract | ectopic

(Evans and Bashaw, 2010)

tract | embryonic stage

(Loy et al., 2025)

vMP2 neuron

(Hiramoto and Hiromi, 2006, Fritz and VanBerkum, 2002, Fritz and VanBerkum, 2000)

Detailed Description

Statement

Reference

The adult sLNv neurons of robo1¹/robo1^BG01092 transheterozygotes do not show significant axon length differences compared to controls.

(Oliva et al., 2016)

robo1¹ homozygous embryonic heart cardioblasts show a rounded morphology, show significant decreases in migration velocity, as well as in filopodial and lamellopodial extensions and activities, as compared to controls; these defects are associated with embryonic heart lumen formation defects, as compared to controls. robo1¹ heterozygous cardioblasts also show a significant decrease in migration velocity, filopodial activity and lamellopodial activity, as compared to controls.

(Raza and Jacobs, 2016)

robo1¹ homozygous embryos display defects in midline repulsion leading to axons ectopically crossing the ventral nerve cord midline.

(Reichert et al., 2016)

robo1¹/robo1¹, but not robo1¹/+, mutant embryos exhibit ventral nerve cord defects, including axon guidance defects, with FasII-positive axons crossing the midline in every segment, and the pCC neuron inappropriately extends across the midline, in contrast to wild type.

(Brown et al., 2015)

Axons from the medial Fas2-positive pathway cross the line in every segment in mutant embryos.

(Evans and Bashaw, 2012)

robo¹/robo² embryos exhibit gonad compaction defects and germ cell ensheathment defects.

(Weyers et al., 2011)

In robo¹ mutants, the medial Fas2-positive axon tracts ectopically cross and recross the midline. The axons expressing Scer\GAL4^ap-md544 also collapse along the midline and follow the medial Fas2-tract.

(Evans and Bashaw, 2010)

Fas2-positive longitudinal axons show repeated crossing of the midline in robo¹/robo⁸ stage 16 embryos.

99.1% of segments have Fas2-positive axons extending across or along the midline in stage 16-17 homozygous embryos.

(Spitzweck et al., 2010)

Homozygous robo¹ mutant embryos display a phenotype where axons repeatedly cross the midline in a circular fashion.

(Wright et al., 2010)

Single cell robo¹ leg motoneuron clones induced 48 hr after hatching have the following phenotypes: the posterior dendritic branch crosses the midline at the same site as it does in wild type clones, but there are ectopic branches the that extend toward the midline at more anterior positions within the neuropil; more of the dendritic arborization is found in the medial territory than controls; the mean centre of mass is shifted toward the midline compared to controls; the mean 33rd percentile of arborization is shifted towards the midline.

Single cell robo¹ leg motoneuron clones induced 96 hr after hatching have the following phenotypes: a shift in the distribution of dendrites toward the midline; the mean centre of mass shifts towards the midline compared to controls; the mean 33rd percentile of arborization shifts towards the midline. There is no evidence for a disruption to the axonal projections of clones to the periphery. In a dorsoventral plane, these clones show a shift of dendrites towards the posterior neuropil and a slight loss in the ventral neuropil.

(Brierley et al., 2009)

robo¹/robo² mutants exhibit an ectopic midline innervation phenotype (with 100% penetrance for MN-LL1 and 89% penetrance for MN-DA3).

(Mauss et al., 2009)

robo¹ mutants exhibit axon guidance defects whereby the commissural axons cross the midline repetitively to give characteristic 'roundabouts'.

robo¹ heterozygous mutants appear wild-type.

(Berni et al., 2008)

Many of the dorsal abdominal clusters in robo¹ mutant animals have branches that exceed the normal level of extension at approximately 21 and 22 hours after egg laying. The maximal dendrite length with respect to the most dorsally positioned neuronal cell body is significantly altered in mutants.

Class IV robo¹ mutant neurons display dendrite over-elongation and reduced branching. Class IV neurons show less high order branches and form longer dendrite process compared to control embryos. The number of branches of class IV neurons is significantly decreased in robo¹ mutants at 22 hours after egg laying. The dorsal elongation and the average branch length are significantly increased.

Class I neurons in robo¹ mutant embryos do not show any defects in dendrite branch length or number compared to control embryos.

robo¹ single cell mutant class IV neurons have less high order branches than control clones. The number of quaternary and higher order branches is less than half compared to controls. The average length of all branch orders is unchanged.

robo¹ single cell mutant clones of either two dorsal class I neurons display normal dendrite morphology.

(Dimitrova et al., 2008)

robo¹ embryos show an axon guidance defect of the Apterous neurons with neurons aberrantly crossing the midline. robo¹ Apterous neurons exhibit these crossing defects during the initial extension toward the midline and throughout development.

(Garbe et al., 2007)

Medial axon bundles abnormally cross and re-cross the midline in homozygous robo¹ mutant embryos.

(Garbe et al., 2007)

robo¹ mutant embryos present a fused commissure phenotype in 100% of neuromeres.

(Simionato et al., 2007)

The initial lateral projection and posterior turn of the dMP2 and MP1 pioneer neurons are normal in developing mutant embryos. The axons fasciculate as in wild type and extend their axons posteriorly. When they reach the commissure of the next segment, MP1 crosses the commissure and follows the correct longitudinal pathway. However, most dMP2 axons show an abnormal trajectory at this point, separating from the MP1 axon, tracking the commissural axons and crossing the midline (rather than following a longitudinal pathway as in wild type).

The normal anterior projections of the pCC and vMP2 neurons are misdirected contralaterally in mutant embryos.

(Hiramoto and Hiromi, 2006)

robo¹ embryos show weak salivary gland guidance defects.

(Kolesnikov and Beckendorf, 2005)

Subtle myocardial cell alignment defects are observed in lea^robo2-8 stage 16 embryos.

(Qian et al., 2005)

In robo¹/robo¹ embryos there is ectopic midline crossing of most CNS axons, and disorganized and inappropriate serotonergic neuron branching towards the midline. Despite their defects in serotonergic neuronal axon guidance, these neurons retain their normal ability to take up serotonin.

(Couch et al., 2004)

Mutant embryos do not show defects in the position of the vmd1a neuron of the ventral multidendritic neuron cluster in the abdomen; a single vmd1a neuron is seen in each hemisegment.

(Orgogozo et al., 2004)

About 26-40% of heterozygous robo¹ stage 16 embryos exhibit axon bundles that cross the midline incorrectly.

(Hsouna et al., 2003)

Homozygous embryos show sensory axon defects; one or more axons from the lch5 cluster project inappropriately to join the v'ch1 axon or the v' cluster in 10% of hemisegments. In these hemisegments, the remaining lch5 axons project in a normal fashion to the intersegmental nerve. In addition, the axon of one of the lesA and ldaA neurons (usually the lesA neuron) follows the v'ch1 pathway in 23% of hemisegments. Occasionally (3% of hemisegments), v'ch1 or other v' cluster axons grow dorsally before turning back into the segmental nerve or inappropriately joining the intersegmental nerve. No defects are seen in the dorsal trunk, transverse connective or visceral branch.

(Parsons et al., 2003)

The terminal branches of the dbd neurons generally cross the midline and often form mirror image bilateral projections. The projections retain their association with the medial Fas2 fascicle. Approximately half of the time the terminal branches of the ch neurons have a branch crossing the midline. The projections retain their association with the intermediate Fas2 fascicle.

(Zlatic et al., 2003)

In robo¹/+ embryos, midline crossovers are seen in the pCC/MP2 pathway axons in 25% of embryos. An average of 1.1 crossovers are seen per embryo.

(Fritz and VanBerkum, 2002)

One or two Fas2-positive axon bundles cross the midline in about 26% of heterozygous embryos.

(Kim et al., 2002)

robo¹/+ heterozygous mutant embryos exhibit midline crossing at a frequency of ~4.8%.

(Ratnaparkhi et al., 2002)

43% of heterozygous embryos show abnormal midline crossing of axons.

(Kim et al., 2001)

5% of segments show crossing of the midline by muscles 6/7, 11% show abnormal insertion of muscle 5 and 15% show abnormal insertion of muscles 6/7 in mutant embryos.

(Kramer et al., 2001)

Approximately 53% of heterozygous embryos show periodic crossovers of Fas2-positive axons across the midline.

(Fritz and VanBerkum, 2000)

In robo¹ mutant embryos all of the innermost fascicles from each side of the midline combine to form a single thick bundle that meanders back and forth along the midline.

(Rajagopalan et al., 2000)

The inner Fas2-positive longitudinal bundle crosses the midline and circles around it in the central nervous system of homozygous embryos.

(Sun et al., 2000)

Embryos heterozygous for either robo¹ or sli² show deviation of longitudinal fascicles towards the midline in less than 5% of segments.

(Battye et al., 1999)

The ap-expressing interneurons cross the midline in every segment in homozygous embryos, join up with their contralateral homologues, and often project anteriorly in one discrete longitudinal fascicle. In heterozygous embryos 5% of all ap-expressing axons cross the midline.

(Kidd et al., 1998)

Mutant phenotype of lateral chordotonal axons includes: shorter axons or defasciculated axons.

(Kolodziej et al., 1995)

"Fuzzy commisure" phenotype in embryonic central nervous system.

(Seeger et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

robo1¹/robo1[+], sli² has abnormal neuroanatomy phenotype, enhanceable by robo[+]/β-Spec^em6

(Garbe et al., 2007)

robo1¹/robo1[+], sli² has abnormal neuroanatomy phenotype, enhanceable by robo[+]/β-Spec^G0074

(Garbe et al., 2007)

robo1¹/robo1[+], sli² has abnormal neuroanatomy phenotype, enhanceable by robo[+]/β-Spec^G0198

(Garbe et al., 2007)

chb⁴, robo1¹ has abnormal neuroanatomy phenotype, enhanceable by robo2⁸

(Lee et al., 2004)

robo1¹ has abnormal neuroanatomy | embryonic stage 16 | dominant phenotype, enhanceable by sli[+]/sli¹

(Hsouna et al., 2003)

robo1¹ has abnormal neuroanatomy | embryonic stage 16 | dominant phenotype, enhanceable by Abl⁴/Abl[+]

(Hsouna et al., 2003)

robo1¹ has abnormal neuroanatomy | embryonic stage 16 | dominant phenotype, enhanceable by Abl⁴

(Hsouna et al., 2003)

robo1¹, sli[+]/sli¹ has abnormal neuroanatomy | embryonic stage 16 | dominant phenotype, enhanceable by Abl⁴/Abl[+]

(Hsouna et al., 2003)

robo1¹ has abnormal neuroanatomy | embryonic stage 16 | dominant phenotype, enhanceable by Abl^UAS.cHa/Scer\GAL4^ftz.ng

(Hsouna et al., 2003)

robo1¹ has abnormal neuroanatomy | embryonic stage 16 | dominant phenotype, enhanceable by Abl^KN.UAS/Scer\GAL4^ftz.ng

(Hsouna et al., 2003)

robo1¹ has abnormal neuroanatomy | embryonic stage 16 | dominant phenotype, enhanceable by Abl::Hsap\ABL1::Hsap\BCR^P210.UAS/Scer\GAL4^ftz.ng

(Hsouna et al., 2003)

robo1¹ has abnormal neuroanatomy | embryonic stage 16 | dominant phenotype, enhanceable by Abl::Hsap\ABL1::Hsap\BCR^KR.UAS/Scer\GAL4^ftz.ng

(Hsouna et al., 2003)

robo1¹ has abnormal neuroanatomy phenotype, enhanceable by Ggal\MLCK^ct.UAS/Scer\GAL4^ftz.ng

(Kim et al., 2002)

robo1¹ has abnormal neuroanatomy phenotype, enhanceable by Gαq^Q203L.UAS/Scer\GAL4^ftz.ng

(Ratnaparkhi et al., 2002)

robo1¹, lea[+]/robo2⁸ has abnormal neuroanatomy | recessive phenotype, enhanceable by capt^k01217/capt[+]

(Wills et al., 2002)

NOT Enhanced by

Statement

Reference

robo1¹ has abnormal neuroanatomy | embryonic stage | recessive phenotype, non-enhanceable by comm^Δe39/comm^Δe39

(Daiber et al., 2021)

robo1¹ has abnormal cell migration | embryonic stage | dominant phenotype, non-enhanceable by robo2^lea-2/robo2^lea-2

(Raza and Jacobs, 2016)

robo1¹ has abnormal cell shape | embryonic stage phenotype, non-enhanceable by robo2^lea-2/robo2^lea-2

(Raza and Jacobs, 2016)

robo1¹, sli² has abnormal neuroanatomy | embryonic stage 16 phenotype, non-enhanceable by Nedd4^UAS.Tag:MYC/Scer\GAL4^elav.PLu

(Keleman et al., 2005)

robo1¹, sli² has abnormal neuroanatomy | embryonic stage 16 phenotype, non-enhanceable by Scer\GAL4^insc-Mz1407/Nedd4^UAS.Tag:MYC

(Keleman et al., 2005)

robo1¹, sli² has abnormal neuroanatomy | embryonic stage 16 phenotype, non-enhanceable by Nedd4^EY00500/Scer\GAL4^elav.PLu

(Keleman et al., 2005)

robo1¹, sli² has abnormal neuroanatomy | embryonic stage 16 phenotype, non-enhanceable by Scer\GAL4^insc-Mz1407/Nedd4^EY00500

(Keleman et al., 2005)

robo1¹, sli² has abnormal neuroanatomy | embryonic stage 16 phenotype, non-enhanceable by Df(3R)ED4688

(Keleman et al., 2005)

chb⁴, robo1¹ has abnormal neuroanatomy phenotype, non-enhanceable by robo3¹

(Lee et al., 2004)

robo1¹, robo3[+]/robo3¹ has abnormal neuroanatomy | recessive phenotype, non-enhanceable by capt¹⁰/capt[+]

(Wills et al., 2002)

Suppressed by

Statement

Reference

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, suppressible | partially by Mmus\Robo1^{10xUAS.Tag:HA,Tag:SS(wg)}/Scer\GAL4^elav.PLu

(Loy et al., 2025)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, suppressible | partially by Scer\GAL4^elav.PLu/Mmus\Robo2^{10xUAS.Tag:HA,Tag:SS(wg)}

(Loy et al., 2025)

robo1¹, sli² has abnormal neuroanatomy | embryonic stage phenotype, suppressible | partially by Tace¹⁹

(Zang et al., 2022)

robo1¹, sli² has abnormal neuroanatomy | embryonic stage phenotype, suppressible | partially by Scer\GAL4^elav.PLu/Tace^{10xUAS.Tag:HA}

(Zang et al., 2022)

robo1¹ has increased cell number | somatic clone phenotype, suppressible by fra³/fra³

(de Torres-Jurado et al., 2022)

robo1¹ has abnormal neuroanatomy | recessive | embryonic stage phenotype, suppressible | partially by Cele\sax-3^robo1.Tag:HA

(Daiber et al., 2021)

robo1¹ has abnormal neuroanatomy | recessive | embryonic stage phenotype, suppressible by robo1::Cele\sax-3^{robo1E-sax3PC.Tag:HA}

(Daiber et al., 2021)

robo1¹ has abnormal neuroanatomy | recessive | embryonic stage phenotype, suppressible by robo1::Cele\sax-3^{robo1EP-sax3C.Tag:HA}

(Daiber et al., 2021)

robo1¹ has abnormal neuroanatomy | recessive | embryonic stage phenotype, suppressible by robo1::Cele\sax-3^{robo1EC-sax3P.Tag:HA}

(Daiber et al., 2021)

robo1¹ has abnormal cell migration | dominant | embryonic stage phenotype, suppressible by fra³/fra[+]

(Raza and Jacobs, 2016)

robo1¹ has abnormal cell migration | dominant | embryonic stage phenotype, suppressible by unc-5[+]/unc-5⁸

(Raza and Jacobs, 2016)

robo1¹ has abnormal cell migration | dominant | embryonic stage phenotype, suppressible by Dscam1[+]/Dscam1¹

(Raza and Jacobs, 2016)

robo1¹ has abnormal cell migration | dominant | embryonic stage phenotype, suppressible by Dscam1⁰⁵⁵¹⁸/Dscam1[+]

(Raza and Jacobs, 2016)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, suppressible by robo1::robo3^{1.CC2.3.CC3.robo1.Tag:HA}

(Spitzweck et al., 2010)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, suppressible by robo1::robo3^{3.Ig2.1.Ig3.robo1.Tag:HA}

(Spitzweck et al., 2010)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, suppressible by robo1::robo3^{3.TM.1.CC0.robo1.Tag:HA}

(Spitzweck et al., 2010)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, suppressible by robo1::robo3^{3.CC0.1.CC1.robo1.Tag:HA}

(Spitzweck et al., 2010)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, suppressible by robo1::robo3^{3.CC1.1.CC2.robo1.Tag:HA}

(Spitzweck et al., 2010)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, suppressible by fra::robo1^{SD.UAS.Tag:V5,Tag:polyHis,Tag:HA,Tag:SS(wg)}/Scer\GAL4^insc-Mz1407

(Gilestro, 2008)

β-Spec^em6, robo[+]/robo1¹, sli² has abnormal neuroanatomy phenotype, suppressible | partially by β-Spec^UAS.Tag:MYC/Scer\GAL4^elav.PLu

(Garbe et al., 2007)

robo1¹ has abnormal neuroanatomy | recessive | embryonic stage phenotype, suppressible by fra^{ΔC.UAS.Tag:HA}/Scer\GAL4^elav.PLu

(Garbe et al., 2007)

robo1¹ has abnormal neuroanatomy phenotype, suppressible by elav⁵

(Simionato et al., 2007)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, suppressible by Ts(1Lt;YSt)B118+Ts(1Rt;YLt)T9

(Hiramoto and Hiromi, 2006)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, suppressible by fra³

(Hiramoto and Hiromi, 2006)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, suppressible by Scer\GAL4^15J2/Mmus\Itpr1^{sponge.UAS.GFP}

(Hiramoto and Hiromi, 2006)

robo1¹, sli² has abnormal neuroanatomy | embryonic stage 16 phenotype, suppressible by Scer\GAL4^elav.PLu/ena^{UAS.Tag:polyHis}

(Keleman et al., 2005)

NOT suppressed by

Statement

Reference

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by Scer\GAL4^elav.PLu/Mmus\Robo3^{10xUAS.1.Tag:HA,Tag:SS(wg)}

(Loy et al., 2025)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by Scer\GAL4^elav.PLu/Mmus\Robo3^{10xUAS.2.Tag:HA,Tag:SS(wg)}

(Loy et al., 2025)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by Mmus\Robo1^robo1.Tag:HA

(Loy et al., 2025)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by Mmus\Robo2^robo1.Tag:HA

(Loy et al., 2025)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by Mmus\Robo3^{robo1.1.Tag:HA}

(Loy et al., 2025)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by Mmus\Robo3^{robo1.2.Tag:HA}

(Loy et al., 2025)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by robo1::Mmus\Robo2^{dR1Ig1.robo1.Tag:HA}

(Loy et al., 2025)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by robo1::Mmus\Robo1^{dR1Ig1.robo1.Tag:HA}

(Loy et al., 2025)

robo1¹ has abnormal neuroanatomy | recessive | embryonic stage phenotype, non-suppressible by robo1::Cele\sax-3^{sax3E-robo1PC.Tag:HA}

(Daiber et al., 2021)

robo1¹ has abnormal neuroanatomy | recessive | embryonic stage phenotype, non-suppressible by robo1::Cele\sax-3^{sax3EC-robo1P.Tag:HA}

(Daiber et al., 2021)

robo1¹ has abnormal neuroanatomy | recessive | embryonic stage phenotype, non-suppressible by robo1::Cele\sax-3^{sax3EP-robo1C.Tag:HA}

(Daiber et al., 2021)

robo1¹ has abnormal neuroanatomy | recessive | embryonic stage phenotype, non-suppressible by comm^Δe39/comm^Δe39

(Daiber et al., 2021)

robo1¹, robo2^lea-2 has abnormal cell migration | dominant | embryonic stage phenotype, non-suppressible by Scer\GAL4^Mef2.247/robo1^UAS.cKa

(Raza and Jacobs, 2016)

robo1¹ has abnormal cell migration | dominant | embryonic stage phenotype, non-suppressible by Scer\GAL4^Mef2.247/fra^UAS.cKa

(Raza and Jacobs, 2016)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by robo2^robo1.Tag:HA

(Spitzweck et al., 2010)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by robo3^robo1.Tag:HA

(Spitzweck et al., 2010)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by robo1::robo3^{1.Ig2.3.Ig3.robo1.Tag:HA}

(Spitzweck et al., 2010)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by robo1::robo3^{1.TM.3.CC0.robo1.Tag:HA}

(Spitzweck et al., 2010)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by robo1::robo3^{1.CC0.3.CC1.robo1.Tag:HA}

(Spitzweck et al., 2010)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by robo1::robo3^{1.CC1.3.CC2.robo1.Tag:HA}

(Spitzweck et al., 2010)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by robo1::robo3^{3.CC2.1.CC3.robo1.Tag:HA}

(Spitzweck et al., 2010)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by fra^{UAS.Tag:HA.cGa}/Scer\GAL4^insc-Mz1407

(Gilestro, 2008)

robo1¹ has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by Scer\GAL4^15J2/Mmus\Itpr1^{mut.sponge.UAS.GFP}

(Hiramoto and Hiromi, 2006)

Enhancer of

Statement

Reference

robo1¹ is an enhancer of abnormal neuroanatomy phenotype of Scer\GAL4^eg-Mz360, fra^{ΔC.UAS.Tag:HA}

(O'Donnell and Bashaw, 2013)

robo1¹ is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of robo3¹

(Evans and Bashaw, 2010)

sli², robo[+], robo1¹ is an enhancer of abnormal neuroanatomy phenotype of β-Spec^em6

(Garbe et al., 2007)

sli², robo[+], robo1¹ is an enhancer of abnormal neuroanatomy phenotype of β-Spec^G0074

(Garbe et al., 2007)

sli², robo[+], robo1¹ is an enhancer of abnormal neuroanatomy phenotype of β-Spec^G0198

(Garbe et al., 2007)

robo[+]/robo1¹ is an enhancer of abnormal neuroanatomy | embryonic stage 16 phenotype of Abl⁴

(Hsouna et al., 2003)

robo[+]/robo1¹ is an enhancer of abnormal neuroanatomy phenotype of Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng

(Kim et al., 2002)

robo[+]/robo1¹ is an enhancer of abnormal neuroanatomy phenotype of Gαq^Q203L.UAS, Scer\GAL4^ftz.ng

(Ratnaparkhi et al., 2002)

NOT Enhancer of

Statement

Reference

robo1¹/robo1[+] is a non-enhancer of abnormal cell migration | embryonic stage phenotype of Dscam1⁰⁵⁵¹⁸/Dscam1¹

(Raza and Jacobs, 2016)

Suppressor of

Statement

Reference

robo1¹/robo1¹ is a suppressor of increased cell number | somatic clone phenotype of fra³

(de Torres-Jurado et al., 2022)

robo1¹/robo1[+] is a suppressor of abnormal cell migration | dominant | embryonic stage phenotype of fra³

(Raza and Jacobs, 2016)

robo1¹/robo1[+] is a suppressor | partially of abnormal cell migration | dominant | embryonic stage phenotype of unc-5⁸

(Raza and Jacobs, 2016)

robo[+], Df(2R)BSC482, robo1¹, + is a suppressor of abnormal neuroanatomy phenotype of Trim9⁴⁶

(Song et al., 2011)

robo1¹ is a suppressor of abnormal neuroanatomy | recessive | embryonic stage phenotype of fra^unspecified

(Yang et al., 2009)

robo1¹ is a suppressor | partially of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^elav.PLu, fra^{ΔC.UAS.Tag:HA}, fra^unspecified

(Garbe et al., 2007)

robo[+]/robo1¹ is a suppressor of abnormal neuroanatomy phenotype of elav⁵

(Simionato et al., 2007)

NOT Suppressor of

Statement

Reference

robo1¹ is a non-suppressor of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^ap-md544, robo2^{UAS.Tag:HA,Tag:SS(wg)}

(Evans and Bashaw, 2010)

robo1¹/robo3¹ is a non-suppressor of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^ap-md544, robo2^{UAS.Tag:HA,Tag:SS(wg)}

(Evans and Bashaw, 2010)

robo1¹/robo1¹ is a non-suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^elav.PLu, unc-5^{UAS.Tag:HA,Tag:SS(wg)}

(Keleman and Dickson, 2001)

robo1⁸/robo1¹ is a non-suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^ap-md544, unc-5^{UAS.Tag:HA,Tag:SS(wg)}

(Keleman and Dickson, 2001)

Other

Statement

Reference

robo1¹, sli² has abnormal neuroanatomy | embryonic stage phenotype

(Zang et al., 2022)

comm^Δe39, robo1¹, robo1^Tag:HA has abnormal neuroanatomy | embryonic stage phenotype

(Daiber et al., 2021)

Cele\sax-3^{10xUAS.Tag:HA,Tag:SS(wg)}, Scer\GAL4^elav.PLu, robo1¹ has abnormal neuroanatomy | embryonic stage phenotype

(Daiber et al., 2021)

Cele\sax-3^robo1.Tag:HA, comm^Δe39, robo1¹ has abnormal neuroanatomy | recessive | embryonic stage phenotype

(Daiber et al., 2021)

Scer\GAL4^elav.PLu, robo1^{ΔFn1-3.Tag:HA}, robo1¹, robo2^{UAS.Tag:HA,Tag:SS(wg)} has abnormal neuroanatomy | embryonic stage phenotype

(Brown and Evans, 2020)

Scer\GAL4^elav.PLu, robo1^ΔFn1.Tag:HA, robo1¹, robo2^{UAS.Tag:HA,Tag:SS(wg)} has abnormal neuroanatomy | embryonic stage phenotype

(Brown and Evans, 2020)

Scer\GAL4^elav.PLu, robo1^ΔFn2.Tag:HA, robo1¹, robo2^{UAS.Tag:HA,Tag:SS(wg)} has abnormal neuroanatomy | embryonic stage phenotype

(Brown and Evans, 2020)

Scer\GAL4^elav.PLu, robo1^ΔFn3.Tag:HA, robo1¹, robo2^{UAS.Tag:HA,Tag:SS(wg)} has abnormal neuroanatomy | embryonic stage phenotype

(Brown and Evans, 2020)

Scer\GAL4^elav.PLu, robo1^{ΔIg2ΔFn3.Tag:HA}, robo1¹, robo2^{UAS.Tag:HA,Tag:SS(wg)} has abnormal neuroanatomy | embryonic stage phenotype

(Brown and Evans, 2020)

Scer\GAL4^elav.PLu, robo1^{ΔIg2-5.Tag:HA}, robo1¹, robo2^{UAS.Tag:HA,Tag:SS(wg)} has abnormal neuroanatomy | embryonic stage phenotype

(Brown and Evans, 2020)

Scer\GAL4^elav.PLu, robo1¹, robo1^Tag:HA, robo2^{UAS.Tag:HA,Tag:SS(wg)} has abnormal neuroanatomy | embryonic stage phenotype

(Brown and Evans, 2020)

robo1¹, robo2^lea-2 has abnormal cell adhesion | embryonic stage phenotype

(Raza and Jacobs, 2016)

robo1¹/robo1[+], sli² has abnormal neuroanatomy phenotype

(Garbe et al., 2007)

robo1¹, sli² has abnormal neuroanatomy | embryonic stage 16 phenotype

(Keleman et al., 2005)

chb⁴, robo1¹ has abnormal neuroanatomy phenotype

(Lee et al., 2004)

robo1¹, lea[+]/robo2⁸ has abnormal neuroanatomy phenotype

(Lee et al., 2004)

chb⁴/chb[+], robo1¹ has abnormal neuroanatomy phenotype

(Lee et al., 2004)

robo1¹, robo2⁸ has abnormal neuroanatomy phenotype

(Lee et al., 2004)

robo1¹, robo3¹ has abnormal neuroanatomy phenotype

(Orgogozo et al., 2004)

Abl⁴, robo1¹ has abnormal neuroanatomy | embryonic stage 16 phenotype

(Hsouna et al., 2003)

robo1¹, lea[+]/robo2⁸ has abnormal neuroanatomy | recessive phenotype

(Wills et al., 2002)

robo1¹, robo3[+]/robo3¹ has abnormal neuroanatomy | recessive phenotype

(Wills et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

robo1¹ has embryonic heart cardioblast | embryonic stage phenotype, enhanceable by fra³/fra[+]

(Raza and Jacobs, 2016)

robo1¹ has filopodium | embryonic stage phenotype, enhanceable by fra³/fra[+]

(Raza and Jacobs, 2016)

robo1¹ has lamellipodium | embryonic stage phenotype, enhanceable by fra³/fra[+]

(Raza and Jacobs, 2016)

robo1¹ has embryonic heart cardioblast | embryonic stage phenotype, enhanceable by unc-5[+]/unc-5⁸

(Raza and Jacobs, 2016)

robo1¹ has filopodium | embryonic stage phenotype, enhanceable by unc-5[+]/unc-5⁸

(Raza and Jacobs, 2016)

robo1¹ has lamellipodium | embryonic stage phenotype, enhanceable by unc-5[+]/unc-5⁸

(Raza and Jacobs, 2016)

robo1¹ has embryonic heart cardioblast | embryonic stage phenotype, enhanceable by Dscam1[+]/Dscam1¹

(Raza and Jacobs, 2016)

robo1¹ has filopodium | embryonic stage phenotype, enhanceable by Dscam1[+]/Dscam1¹

(Raza and Jacobs, 2016)

robo1¹ has lamellipodium | embryonic stage phenotype, enhanceable by Dscam1[+]/Dscam1¹

(Raza and Jacobs, 2016)

robo1¹/robo1[+], sli² has medial longitudinal fascicle | ectopic phenotype, enhanceable by robo[+]/β-Spec^em6

(Garbe et al., 2007)

robo1¹/robo1[+], sli² has medial longitudinal fascicle | ectopic phenotype, enhanceable by robo[+]/β-Spec^G0074

(Garbe et al., 2007)

robo1¹/robo1[+], sli² has medial longitudinal fascicle | ectopic phenotype, enhanceable by robo[+]/β-Spec^G0198

(Garbe et al., 2007)

robo1¹ has presumptive embryonic salivary gland phenotype, enhanceable by robo2¹

(Kolesnikov and Beckendorf, 2005)

robo1¹ has dorsal vessel wall cell phenotype, enhanceable by robo2⁸

(Qian et al., 2005)

chb⁴, robo1¹ has larval neuron phenotype, enhanceable by robo2⁸

(Lee et al., 2004)

robo1¹ has larval longitudinal connective | embryonic stage 16 phenotype, enhanceable by sli[+]/sli¹

(Hsouna et al., 2003)

robo1¹ has larval longitudinal connective | embryonic stage 16 phenotype, enhanceable by Abl⁴/Abl[+]

(Hsouna et al., 2003)

robo1¹ has larval longitudinal connective | embryonic stage 16 phenotype, enhanceable by Abl⁴

(Hsouna et al., 2003)

robo1¹, sli[+]/sli¹ has larval longitudinal connective | embryonic stage 16 phenotype, enhanceable by Abl⁴/Abl[+]

(Hsouna et al., 2003)

robo1¹ has larval longitudinal connective | embryonic stage 16 phenotype, enhanceable by Abl^UAS.cHa/Scer\GAL4^ftz.ng

(Hsouna et al., 2003)

robo1¹ has larval longitudinal connective | embryonic stage 16 phenotype, enhanceable by Abl^KN.UAS/Scer\GAL4^ftz.ng

(Hsouna et al., 2003)

robo1¹ has larval longitudinal connective | embryonic stage 16 phenotype, enhanceable by Abl::Hsap\ABL1::Hsap\BCR^P210.UAS/Scer\GAL4^ftz.ng

(Hsouna et al., 2003)

robo1¹ has larval longitudinal connective | embryonic stage 16 phenotype, enhanceable by Abl::Hsap\ABL1::Hsap\BCR^KR.UAS/Scer\GAL4^ftz.ng

(Hsouna et al., 2003)

robo1¹ has dMP2 neuron phenotype, enhanceable by Rac1^V12.UAS/Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

robo1¹ has pCC neuron phenotype, enhanceable by Rac1^V12.UAS/Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

robo1¹ has vMP2 neuron phenotype, enhanceable by Rac1^V12.UAS/Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

robo1¹ has dMP2 neuron phenotype, enhanceable by Rho1^N19.UAS/Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

robo1¹ has vMP2 neuron phenotype, enhanceable by Rho1^N19.UAS/Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

robo1¹ has pCC neuron phenotype, enhanceable by Rho1^N19.UAS/Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

robo1¹ has dMP2 neuron phenotype, enhanceable by Rho1^V14.UAS/Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

robo1¹ has pCC neuron phenotype, enhanceable by Rho1^V14.UAS/Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

robo1¹ has vMP2 neuron phenotype, enhanceable by Rho1^V14.UAS/Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

robo1¹ has larval longitudinal connective phenotype, enhanceable by Ggal\MLCK^ct.UAS/Scer\GAL4^ftz.ng

(Kim et al., 2002)

robo1¹ has symmetrical commissure | embryonic stage phenotype, enhanceable by Gαq^Q203L.UAS/Scer\GAL4^ftz.ng

(Ratnaparkhi et al., 2002)

robo1¹ has fascicle | embryonic stage phenotype, enhanceable by Gαq^Q203L.UAS/Scer\GAL4^ftz.ng

(Ratnaparkhi et al., 2002)

robo1¹, lea[+]/robo2⁸ has presumptive embryonic/larval central nervous system phenotype, enhanceable by capt^k01217/capt[+]

(Wills et al., 2002)

robo1¹ has larval longitudinal connective phenotype, enhanceable by Ggal\Cam^{KA.ftz.Tag:MT(Khc)}

(Fritz and VanBerkum, 2000)

robo1¹ has larval DA1 motor neuron phenotype, enhanceable by robo2⁵

(Rajagopalan et al., 2000)

robo1¹ has larval muscle system phenotype, enhanceable by robo2⁵

(Rajagopalan et al., 2000)

robo1¹ has pCC neuron phenotype, enhanceable by robo2⁵

(Rajagopalan et al., 2000)

robo1¹ has larval longitudinal connective phenotype, enhanceable by sli[+]/sli^E-158

(Battye et al., 1999)

NOT Enhanced by

Statement

Reference

robo1¹ has embryonic midline of ventral nerve cord phenotype, non-enhanceable by comm^Δe39/comm^Δe39

(Daiber et al., 2021)

robo1¹ has embryonic heart cardioblast phenotype, non-enhanceable by robo2^lea-2/robo2^lea-2

(Raza and Jacobs, 2016)

robo1¹ has lamellipodium | embryonic stage phenotype, non-enhanceable by robo2^lea-2/robo2^lea-2

(Raza and Jacobs, 2016)

robo1¹ has filopodium | embryonic stage phenotype, non-enhanceable by robo2^lea-2/robo2^lea-2

(Raza and Jacobs, 2016)

robo1¹ has embryonic heart cardioblast | embryonic stage phenotype, non-enhanceable by Dscam1⁰⁵⁵¹⁸/Dscam1[+]

(Raza and Jacobs, 2016)

robo1¹ has filopodium | embryonic stage phenotype, non-enhanceable by Dscam1⁰⁵⁵¹⁸/Dscam1[+]

(Raza and Jacobs, 2016)

robo1¹ has lamellipodium | embryonic stage phenotype, non-enhanceable by Dscam1⁰⁵⁵¹⁸/Dscam1[+]

(Raza and Jacobs, 2016)

robo1²/robo1¹ has gonad | embryonic stage phenotype, non-enhanceable by robo2⁴/robo2⁸

(Weyers et al., 2011)

robo1²/robo1¹ has germline cell | embryonic stage phenotype, non-enhanceable by robo2⁴/robo2⁸

(Weyers et al., 2011)

robo1²/robo1¹ has gonad | embryonic stage phenotype, non-enhanceable by robo3¹/robo3¹

(Weyers et al., 2011)

robo1²/robo1¹ has germline cell | embryonic stage phenotype, non-enhanceable by robo3¹/robo3¹

(Weyers et al., 2011)

robo1¹ has embryonic abdominal dorsal sensory organ precursor cluster phenotype, non-enhanceable by robo2⁸

(Dimitrova et al., 2008)

robo1¹ has dendrite phenotype, non-enhanceable by robo2⁸

(Dimitrova et al., 2008)

robo1¹, sli² has larval longitudinal connective | embryonic stage 16 phenotype, non-enhanceable by Nedd4^UAS.Tag:MYC/Scer\GAL4^elav.PLu

(Keleman et al., 2005)

robo1¹, sli² has commissure | embryonic stage 16 phenotype, non-enhanceable by Nedd4^UAS.Tag:MYC/Scer\GAL4^elav.PLu

(Keleman et al., 2005)

robo1¹, sli² has larval longitudinal connective | embryonic stage 16 phenotype, non-enhanceable by Scer\GAL4^insc-Mz1407/Nedd4^UAS.Tag:MYC

(Keleman et al., 2005)

robo1¹, sli² has commissure | embryonic stage 16 phenotype, non-enhanceable by Scer\GAL4^insc-Mz1407/Nedd4^UAS.Tag:MYC

(Keleman et al., 2005)

robo1¹, sli² has larval longitudinal connective | embryonic stage 16 phenotype, non-enhanceable by Nedd4^EY00500/Scer\GAL4^elav.PLu

(Keleman et al., 2005)

robo1¹, sli² has commissure | embryonic stage 16 phenotype, non-enhanceable by Nedd4^EY00500/Scer\GAL4^elav.PLu

(Keleman et al., 2005)

robo1¹, sli² has larval longitudinal connective | embryonic stage 16 phenotype, non-enhanceable by Scer\GAL4^insc-Mz1407/Nedd4^EY00500

(Keleman et al., 2005)

robo1¹, sli² has commissure | embryonic stage 16 phenotype, non-enhanceable by Scer\GAL4^insc-Mz1407/Nedd4^EY00500

(Keleman et al., 2005)

robo1¹, sli² has larval longitudinal connective | embryonic stage 16 phenotype, non-enhanceable by Df(3R)ED4688

(Keleman et al., 2005)

robo1¹, sli² has commissure | embryonic stage 16 phenotype, non-enhanceable by Df(3R)ED4688

(Keleman et al., 2005)

chb⁴, robo1¹ has larval neuron phenotype, non-enhanceable by robo3¹

(Lee et al., 2004)

robo1¹ has dMP2 neuron phenotype, non-enhanceable by Scer\GAL4^ftz.ng/Cdc42^V12.UAS

(Fritz and VanBerkum, 2002)

robo1¹ has pCC neuron phenotype, non-enhanceable by Scer\GAL4^ftz.ng/Cdc42^V12.UAS

(Fritz and VanBerkum, 2002)

robo1¹ has vMP2 neuron phenotype, non-enhanceable by Scer\GAL4^ftz.ng/Cdc42^V12.UAS

(Fritz and VanBerkum, 2002)

robo1¹ has dMP2 neuron phenotype, non-enhanceable by Rac1^N17.UAS/Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

robo1¹ has pCC neuron phenotype, non-enhanceable by Rac1^N17.UAS/Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

robo1¹ has vMP2 neuron phenotype, non-enhanceable by Rac1^N17.UAS/Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

robo1¹, robo3[+]/robo3¹ has presumptive embryonic/larval central nervous system phenotype, non-enhanceable by capt¹⁰/capt[+]

(Wills et al., 2002)

robo1¹ has phenotype, non-enhanceable by Sos[+]/Sos^e49

(Fritz and VanBerkum, 2000)

robo1¹ has presumptive embryonic/larval nervous system phenotype, non-enhanceable by robo3¹

(Rajagopalan et al., 2000)

robo1¹ has larval muscle system phenotype, non-enhanceable by robo3¹

(Rajagopalan et al., 2000)

Suppressed by

Statement

Reference

robo1¹ has embryonic ventral nerve cord phenotype, suppressible | partially by Mmus\Robo1^{10xUAS.Tag:HA,Tag:SS(wg)}/Scer\GAL4^elav.PLu

(Loy et al., 2025)

robo1¹ has embryonic ventral nerve cord phenotype, suppressible | partially by Scer\GAL4^elav.PLu/Mmus\Robo2^{10xUAS.Tag:HA,Tag:SS(wg)}

(Loy et al., 2025)

robo1¹ has tract | embryonic stage phenotype, suppressible | partially by Mmus\Robo1^{10xUAS.Tag:HA,Tag:SS(wg)}/Scer\GAL4^elav.PLu

(Loy et al., 2025)

robo1¹ has tract | embryonic stage phenotype, suppressible | partially by Scer\GAL4^elav.PLu/Mmus\Robo2^{10xUAS.Tag:HA,Tag:SS(wg)}

(Loy et al., 2025)

robo1¹, sli² has axon | embryonic stage phenotype, suppressible | partially by Tace¹⁹

(Zang et al., 2022)

robo1¹, sli² has fascicle | embryonic stage phenotype, suppressible | partially by Tace¹⁹

(Zang et al., 2022)

robo1¹, sli² has axon | embryonic stage phenotype, suppressible | partially by Scer\GAL4^elav.PLu/Tace^{10xUAS.Tag:HA}

(Zang et al., 2022)

robo1¹, sli² has fascicle | embryonic stage phenotype, suppressible | partially by Scer\GAL4^elav.PLu/Tace^{10xUAS.Tag:HA}

(Zang et al., 2022)

robo1¹ has neuroblast | increased number | somatic clone phenotype, suppressible by fra³/fra³

(de Torres-Jurado et al., 2022)

robo1¹ has embryonic midline of ventral nerve cord phenotype, suppressible | partially by Cele\sax-3^robo1.Tag:HA

(Daiber et al., 2021)

robo1¹ has embryonic midline of ventral nerve cord phenotype, suppressible by robo1::Cele\sax-3^{robo1E-sax3PC.Tag:HA}

(Daiber et al., 2021)

robo1¹ has embryonic midline of ventral nerve cord phenotype, suppressible by robo1::Cele\sax-3^{robo1EC-sax3P.Tag:HA}

(Daiber et al., 2021)

robo1¹ has embryonic midline of ventral nerve cord phenotype, suppressible by robo1::Cele\sax-3^{robo1EP-sax3C.Tag:HA}

(Daiber et al., 2021)

robo1¹ has lamellipodium | embryonic stage phenotype, suppressible by Scer\GAL4^Mef2.247/fra^UAS.cKa

(Raza and Jacobs, 2016)

robo1¹ has filopodium | embryonic stage phenotype, suppressible by Scer\GAL4^Mef2.247/fra^UAS.cKa

(Raza and Jacobs, 2016)

robo1¹ has embryonic heart cardioblast | embryonic stage phenotype, suppressible by fra³/fra[+]

(Raza and Jacobs, 2016)

robo1¹ has embryonic heart cardioblast | embryonic stage phenotype, suppressible by unc-5[+]/unc-5⁸

(Raza and Jacobs, 2016)

robo1¹ has embryonic heart cardioblast | embryonic stage phenotype, suppressible by Dscam1[+]/Dscam1¹

(Raza and Jacobs, 2016)

robo1¹ has embryonic heart cardioblast | embryonic stage phenotype, suppressible by Dscam1⁰⁵⁵¹⁸/Dscam1[+]

(Raza and Jacobs, 2016)

robo1¹, robo2^lea-2 has embryonic heart cardioblast phenotype, suppressible | partially by Scer\GAL4^Mef2.247/robo1^UAS.cKa

(Raza and Jacobs, 2016)

robo1¹, robo2^lea-2 has lamellipodium | embryonic stage phenotype, suppressible | partially by Scer\GAL4^Mef2.247/robo1^UAS.cKa

(Raza and Jacobs, 2016)

robo1¹ has medial longitudinal fascicle phenotype, suppressible by Tcas\robo^{UAS.Tag:HA,Tag:SS(wg)}/Scer\GAL4^elav.PLu

(Evans and Bashaw, 2012)

robo1¹ has medial longitudinal fascicle phenotype, suppressible by Tcas\robo2-3^{UAS.Tag:HA,Tag:SS(wg)}/Scer\GAL4^elav.PLu

(Evans and Bashaw, 2012)

robo1¹ has larval longitudinal connective phenotype, suppressible by robo1::robo3^{1.CC2.3.CC3.robo1.Tag:HA}

(Spitzweck et al., 2010)

robo1¹ has larval longitudinal connective phenotype, suppressible by robo1::robo3^{3.Ig2.1.Ig3.robo1.Tag:HA}

(Spitzweck et al., 2010)

robo1¹ has larval longitudinal connective phenotype, suppressible by robo1::robo3^{3.TM.1.CC0.robo1.Tag:HA}

(Spitzweck et al., 2010)

robo1¹ has larval longitudinal connective phenotype, suppressible by robo1::robo3^{3.CC0.1.CC1.robo1.Tag:HA}

(Spitzweck et al., 2010)

robo1¹ has larval longitudinal connective phenotype, suppressible by robo1::robo3^{3.CC1.1.CC2.robo1.Tag:HA}

(Spitzweck et al., 2010)

robo1¹ has symmetrical commissure | ectopic phenotype, suppressible by fra^{UAS.Tag:HA.cGa}/Scer\GAL4^insc-Mz1407

(Gilestro, 2008)

β-Spec^em6, robo[+]/robo1¹, sli² has medial longitudinal fascicle | ectopic phenotype, suppressible | partially by β-Spec^UAS.Tag:MYC/Scer\GAL4^elav.PLu

(Garbe et al., 2007)

robo1¹ has larval ventral nerve cord commissure | embryonic stage phenotype, suppressible by fra^{ΔC.UAS.Tag:HA}/Scer\GAL4^elav.PLu

(Garbe et al., 2007)

robo1¹ has dMP2 neuron phenotype, suppressible by Ts(1Lt;YSt)B118+Ts(1Rt;YLt)T9

(Hiramoto and Hiromi, 2006)

robo1¹ has dMP2 neuron phenotype, suppressible by fra³

(Hiramoto and Hiromi, 2006)

robo1¹ has pCC neuron phenotype, suppressible by Ts(1Lt;YSt)B118+Ts(1Rt;YLt)T9

(Hiramoto and Hiromi, 2006)

robo1¹ has vMP2 neuron phenotype, suppressible by Ts(1Lt;YSt)B118+Ts(1Rt;YLt)T9

(Hiramoto and Hiromi, 2006)

robo1¹ has dMP2 neuron phenotype, suppressible by Scer\GAL4^15J2/Mmus\Itpr1^{sponge.UAS.GFP}

(Hiramoto and Hiromi, 2006)

robo1¹, sli² has larval longitudinal connective | embryonic stage 16 phenotype, suppressible by Scer\GAL4^elav.PLu/ena^{UAS.Tag:polyHis}

(Keleman et al., 2005)

robo1¹, sli² has commissure | embryonic stage 16 phenotype, suppressible by Scer\GAL4^elav.PLu/ena^{UAS.Tag:polyHis}

(Keleman et al., 2005)

robo1¹ has dMP2 neuron phenotype, suppressible by Cdc42^N17.UAS/Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

robo1¹ has pCC neuron phenotype, suppressible by Cdc42^N17.UAS/Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

robo1¹ has vMP2 neuron phenotype, suppressible by Cdc42^N17.UAS/Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

robo1¹ has larval longitudinal connective phenotype, suppressible by Sos^JC2

(Fritz and VanBerkum, 2000)

Ggal\Cam^{KA.ftz.Tag:MT(Khc)}, robo1¹ has larval longitudinal connective phenotype, suppressible | partially by Sos^JC2

(Fritz and VanBerkum, 2000)

NOT suppressed by

Statement

Reference

robo1¹ has embryonic ventral nerve cord phenotype, non-suppressible by Scer\GAL4^elav.PLu/Mmus\Robo3^{10xUAS.1.Tag:HA,Tag:SS(wg)}

(Loy et al., 2025)

robo1¹ has tract | embryonic stage phenotype, non-suppressible by Scer\GAL4^elav.PLu/Mmus\Robo3^{10xUAS.1.Tag:HA,Tag:SS(wg)}

(Loy et al., 2025)

robo1¹ has embryonic ventral nerve cord phenotype, non-suppressible by Scer\GAL4^elav.PLu/Mmus\Robo3^{10xUAS.2.Tag:HA,Tag:SS(wg)}

(Loy et al., 2025)

robo1¹ has tract | embryonic stage phenotype, non-suppressible by Scer\GAL4^elav.PLu/Mmus\Robo3^{10xUAS.2.Tag:HA,Tag:SS(wg)}

(Loy et al., 2025)

robo1¹ has tract | embryonic stage phenotype, non-suppressible by Mmus\Robo1^robo1.Tag:HA

(Loy et al., 2025)

robo1¹ has embryonic ventral nerve cord phenotype, non-suppressible by Mmus\Robo1^robo1.Tag:HA

(Loy et al., 2025)

robo1¹ has tract | embryonic stage phenotype, non-suppressible by Mmus\Robo2^robo1.Tag:HA

(Loy et al., 2025)

robo1¹ has embryonic ventral nerve cord phenotype, non-suppressible by Mmus\Robo2^robo1.Tag:HA

(Loy et al., 2025)

robo1¹ has tract | embryonic stage phenotype, non-suppressible by Mmus\Robo3^{robo1.1.Tag:HA}

(Loy et al., 2025)

robo1¹ has tract | embryonic stage phenotype, non-suppressible by Mmus\Robo3^{robo1.2.Tag:HA}

(Loy et al., 2025)

robo1¹ has embryonic ventral nerve cord phenotype, non-suppressible by Mmus\Robo3^{robo1.1.Tag:HA}

(Loy et al., 2025)

robo1¹ has embryonic ventral nerve cord phenotype, non-suppressible by Mmus\Robo3^{robo1.2.Tag:HA}

(Loy et al., 2025)

robo1¹ has tract | embryonic stage phenotype, non-suppressible by robo1::Mmus\Robo1^{dR1Ig1.robo1.Tag:HA}

(Loy et al., 2025)

robo1¹ has tract | embryonic stage phenotype, non-suppressible by robo1::Mmus\Robo2^{dR1Ig1.robo1.Tag:HA}

(Loy et al., 2025)

robo1¹ has embryonic ventral nerve cord phenotype, non-suppressible by robo1::Mmus\Robo1^{dR1Ig1.robo1.Tag:HA}

(Loy et al., 2025)

robo1¹ has embryonic ventral nerve cord phenotype, non-suppressible by robo1::Mmus\Robo2^{dR1Ig1.robo1.Tag:HA}

(Loy et al., 2025)

robo1¹ has embryonic midline of ventral nerve cord phenotype, non-suppressible by robo1::Cele\sax-3^{sax3EC-robo1P.Tag:HA}

(Daiber et al., 2021)

robo1¹ has embryonic midline of ventral nerve cord phenotype, non-suppressible by robo1::Cele\sax-3^{sax3E-robo1PC.Tag:HA}

(Daiber et al., 2021)

robo1¹ has embryonic midline of ventral nerve cord phenotype, non-suppressible by robo1::Cele\sax-3^{sax3EP-robo1C.Tag:HA}

(Daiber et al., 2021)

robo1¹ has embryonic midline of ventral nerve cord phenotype, non-suppressible by comm^Δe39/comm^Δe39

(Daiber et al., 2021)

robo1¹ has heart primordium phenotype, non-suppressible by Scer\GAL4^Mef2.247/fra^UAS.cKa

(Raza and Jacobs, 2016)

robo1¹, robo2^lea-2 has filopodium | embryonic stage phenotype, non-suppressible by Scer\GAL4^Mef2.247/robo1^UAS.cKa

(Raza and Jacobs, 2016)

robo1¹, robo2^lea-2 has heart primordium phenotype, non-suppressible by Scer\GAL4^Mef2.247/robo1^UAS.cKa

(Raza and Jacobs, 2016)

robo1¹ has larval longitudinal connective phenotype, non-suppressible by robo3^robo1.Tag:HA

(Spitzweck et al., 2010)

robo1¹ has larval longitudinal connective phenotype, non-suppressible by robo2^robo1.Tag:HA

(Spitzweck et al., 2010)

robo1¹ has larval longitudinal connective phenotype, non-suppressible by robo1::robo3^{1.Ig2.3.Ig3.robo1.Tag:HA}

(Spitzweck et al., 2010)

robo1¹ has larval longitudinal connective phenotype, non-suppressible by robo1::robo3^{1.TM.3.CC0.robo1.Tag:HA}

(Spitzweck et al., 2010)

robo1¹ has larval longitudinal connective phenotype, non-suppressible by robo1::robo3^{1.CC0.3.CC1.robo1.Tag:HA}

(Spitzweck et al., 2010)

robo1¹ has larval longitudinal connective phenotype, non-suppressible by robo1::robo3^{1.CC1.3.CC2.robo1.Tag:HA}

(Spitzweck et al., 2010)

robo1¹ has larval longitudinal connective phenotype, non-suppressible by robo1::robo3^{3.CC2.1.CC3.robo1.Tag:HA}

(Spitzweck et al., 2010)

robo1¹ has symmetrical commissure | ectopic phenotype, non-suppressible by fra::robo1^{SD.UAS.Tag:V5,Tag:polyHis,Tag:HA,Tag:SS(wg)}/Scer\GAL4^insc-Mz1407

(Gilestro, 2008)

robo1¹ has dMP2 neuron phenotype, non-suppressible by Scer\GAL4^15J2/Mmus\Itpr1^{mut.sponge.UAS.GFP}

(Hiramoto and Hiromi, 2006)

robo1¹ has dMP2 neuron phenotype, non-suppressible by Scer\GAL4^ftz.ng/Cdc42^V12.UAS

(Fritz and VanBerkum, 2002)

robo1¹ has dMP2 neuron phenotype, non-suppressible by Rac1^N17.UAS/Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

robo1¹ has pCC neuron phenotype, non-suppressible by Scer\GAL4^ftz.ng/Cdc42^V12.UAS

(Fritz and VanBerkum, 2002)

robo1¹ has pCC neuron phenotype, non-suppressible by Rac1^N17.UAS/Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

robo1¹ has vMP2 neuron phenotype, non-suppressible by Scer\GAL4^ftz.ng/Cdc42^V12.UAS

(Fritz and VanBerkum, 2002)

robo1¹ has vMP2 neuron phenotype, non-suppressible by Rac1^N17.UAS/Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

robo1¹, robo2⁵ has larval ventral nerve cord phenotype, non-suppressible by comm¹

(Rajagopalan et al., 2000)

Enhancer of

Statement

Reference

robo1¹/robo1[+] is an enhancer of embryonic heart cardioblast phenotype of fra³

(Raza and Jacobs, 2016)

robo1¹/robo1[+] is an enhancer of filopodium | embryonic stage phenotype of fra³

(Raza and Jacobs, 2016)

robo1¹/robo1[+] is an enhancer of lamellipodium | embryonic stage phenotype of fra³

(Raza and Jacobs, 2016)

robo1¹/robo1[+] is an enhancer of embryonic heart cardioblast phenotype of unc-5⁸

(Raza and Jacobs, 2016)

robo1¹/robo1[+] is an enhancer of filopodium | embryonic stage phenotype of unc-5⁸

(Raza and Jacobs, 2016)

robo1¹/robo1[+] is an enhancer of embryonic heart cardioblast | embryonic stage phenotype of Dscam1¹

(Raza and Jacobs, 2016)

robo1¹/robo1[+] is an enhancer of filopodium | embryonic stage phenotype of Dscam1¹

(Raza and Jacobs, 2016)

robo1¹/robo1[+] is an enhancer of filopodium | embryonic stage phenotype of Dscam1⁰⁵⁵¹⁸/Dscam1¹

(Raza and Jacobs, 2016)

robo1¹/robo1[+] is an enhancer of lamellipodium | embryonic stage phenotype of Dscam1⁰⁵⁵¹⁸/Dscam1¹

(Raza and Jacobs, 2016)

robo1¹/robo1[+] is an enhancer of embryonic heart cardioblast phenotype of Dscam1⁰⁵⁵¹⁸/Dscam1¹

(Raza and Jacobs, 2016)

robo1¹/robo1[+] is an enhancer of embryonic heart cardioblast phenotype of sli²

(Raza and Jacobs, 2016)

robo1¹/robo1[+] is an enhancer of filopodium | embryonic stage phenotype of sli²

(Raza and Jacobs, 2016)

robo1¹ is an enhancer of larval EW neuron phenotype of Scer\GAL4^eg-Mz360, fra^{ΔC.UAS.Tag:HA}

(O'Donnell and Bashaw, 2013)

robo1¹ is an enhancer of symmetrical commissure phenotype of Scer\GAL4^eg-Mz360, fra^{ΔC.UAS.Tag:HA}

(O'Donnell and Bashaw, 2013)

robo[+], fra³, robo1¹, fra[+] is an enhancer of commissure phenotype of Trim9⁴⁶

(Song et al., 2011)

robo1¹ is an enhancer of larval longitudinal connective phenotype of robo3¹

(Evans and Bashaw, 2010)

sli², robo[+], robo1¹ is an enhancer of medial longitudinal fascicle | ectopic phenotype of β-Spec^em6

(Garbe et al., 2007)

sli², robo[+], robo1¹ is an enhancer of medial longitudinal fascicle | ectopic phenotype of β-Spec^G0074

(Garbe et al., 2007)

sli², robo[+], robo1¹ is an enhancer of medial longitudinal fascicle | ectopic phenotype of β-Spec^G0198

(Garbe et al., 2007)

robo1¹ is an enhancer of presumptive embryonic salivary gland phenotype of robo2¹

(Kolesnikov and Beckendorf, 2005)

robo1¹ is an enhancer of dorsal vessel wall cell phenotype of robo2⁸

(Qian et al., 2005)

robo[+]/robo1¹ is an enhancer of larval longitudinal connective | embryonic stage 16 phenotype of Abl⁴

(Hsouna et al., 2003)

robo1¹ is an enhancer of vMP2 neuron phenotype of Rac1^V12.UAS, Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

robo1¹ is an enhancer of pCC neuron phenotype of Rac1^V12.UAS, Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

robo1¹ is an enhancer of dMP2 neuron phenotype of Rho1^N19.UAS, Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

robo1¹ is an enhancer of vMP2 neuron phenotype of Rho1^N19.UAS, Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

robo1¹ is an enhancer of pCC neuron phenotype of Rho1^N19.UAS, Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

robo1¹ is an enhancer of dMP2 neuron phenotype of Rac1^V12.UAS, Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

robo[+]/robo1¹ is an enhancer of larval longitudinal connective phenotype of Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng

(Kim et al., 2002)

robo[+]/robo1¹ is an enhancer of symmetrical commissure | embryonic stage phenotype of Gαq^Q203L.UAS, Scer\GAL4^ftz.ng

(Ratnaparkhi et al., 2002)

robo[+]/robo1¹ is an enhancer of fascicle | embryonic stage phenotype of Gαq^Q203L.UAS, Scer\GAL4^ftz.ng

(Ratnaparkhi et al., 2002)

robo[+]/robo1¹ is an enhancer of larval ventral nerve cord phenotype of sli^E-158

(Battye et al., 2001)

robo1¹/robo1[+] is an enhancer of larval ventral nerve cord phenotype of sli²

(Battye et al., 2001)

robo[+]/robo1¹ is an enhancer of larval ventral nerve cord phenotype of sli^F81

(Battye et al., 2001)

robo[+]/robo1¹ is an enhancer of larval ventral nerve cord phenotype of sli^GA945

(Battye et al., 2001)

robo[+]/robo1¹ is an enhancer of larval ventral nerve cord phenotype of sli^GA178

(Battye et al., 2001)

robo[+]/robo1¹ is an enhancer of larval ventral nerve cord phenotype of sli¹⁹¹²

(Battye et al., 2001)

robo[+]/robo1¹ is an enhancer of larval ventral nerve cord phenotype of sli⁵⁵⁰

(Battye et al., 2001)

robo[+]/robo1¹ is an enhancer of larval ventral nerve cord phenotype of sli²⁹⁹⁰

(Battye et al., 2001)

robo[+]/robo1¹ is an enhancer of larval ventral nerve cord phenotype of sli⁵³²

(Battye et al., 2001)

robo[+]/robo1¹ is an enhancer of larval ventral nerve cord phenotype of sli³¹⁴⁹

(Battye et al., 2001)

robo[+]/robo1¹ is an enhancer of larval ventral nerve cord phenotype of sli^F119

(Battye et al., 2001)

robo[+]/robo1¹ is an enhancer of larval ventral nerve cord phenotype of sli^GA20

(Battye et al., 2001)

robo[+]/robo1¹ is an enhancer of larval longitudinal connective phenotype of Abl^EP3101, Scer\GAL4^elav.PLu

(Bashaw et al., 2000)

robo1¹ is an enhancer of larval longitudinal connective phenotype of Ggal\Cam^{KA.ftz.Tag:MT(Khc)}

(Fritz and VanBerkum, 2000)

robo1¹ is an enhancer of larval DA1 motor neuron phenotype of robo2⁵

(Rajagopalan et al., 2000)

robo1¹ is an enhancer of larval muscle system phenotype of robo2⁵

(Rajagopalan et al., 2000)

robo1¹ is an enhancer of pCC neuron phenotype of robo2⁵

(Rajagopalan et al., 2000)

robo[+]/robo1¹ is an enhancer of larval longitudinal connective phenotype of sli^E-158

(Battye et al., 1999)

robo1¹/robo1[+] is an enhancer of larval longitudinal connective phenotype of sli²

(Battye et al., 1999)

NOT Enhancer of

Statement

Reference

robo1¹/robo1[+] is a non-enhancer of lamellipodium | embryonic stage phenotype of unc-5⁸

(Raza and Jacobs, 2016)

robo1¹/robo1[+] is a non-enhancer of filopodium | embryonic stage phenotype of Dscam1⁰⁵⁵¹⁸

(Raza and Jacobs, 2016)

robo1¹/robo1[+] is a non-enhancer of lamellipodium | embryonic stage phenotype of Dscam1⁰⁵⁵¹⁸

(Raza and Jacobs, 2016)

robo1¹/robo1[+] is a non-enhancer of embryonic heart cardioblast phenotype of Dscam1⁰⁵⁵¹⁸

(Raza and Jacobs, 2016)

robo1¹/robo1[+] is a non-enhancer of lamellipodium | embryonic stage phenotype of sli²

(Raza and Jacobs, 2016)

robo1²/robo1¹ is a non-enhancer of gonad | embryonic stage phenotype of robo2⁴/robo2⁸

(Weyers et al., 2011)

robo1²/robo1¹ is a non-enhancer of gonadal sheath proper primordium | embryonic stage phenotype of robo2⁴/robo2⁸

(Weyers et al., 2011)

robo1²/robo1¹ is a non-enhancer of germline cell | embryonic stage phenotype of robo2⁴/robo2⁸

(Weyers et al., 2011)

robo1²/robo1¹ is a non-enhancer of gonad | embryonic stage phenotype of Df(2L)Exel7006/robo3¹

(Weyers et al., 2011)

robo1²/robo1¹ is a non-enhancer of germline cell | embryonic stage phenotype of Df(2L)Exel7006/robo3¹

(Weyers et al., 2011)

robo1¹ is a non-enhancer of cephalopharyngeal skeleton phenotype of robo2^S4-14

(Schimmelpfeng et al., 2001)

robo1¹ is a non-enhancer of phenotype of Abl^K417N.UAS, Scer\GAL4^elav.PLu

(Bashaw et al., 2000)

robo1¹ is a non-enhancer of presumptive embryonic/larval nervous system phenotype of robo3¹

(Rajagopalan et al., 2000)

robo1¹ is a non-enhancer of larval muscle system phenotype of robo3¹

(Rajagopalan et al., 2000)

Suppressor of

Statement

Reference

robo1¹/robo1¹ is a suppressor of neuroblast | increased number | somatic clone phenotype of fra³

(de Torres-Jurado et al., 2022)

robo1¹/robo1[+] is a suppressor of embryonic heart cardioblast phenotype of fra³

(Raza and Jacobs, 2016)

robo1¹/robo1[+] is a suppressor | partially of embryonic heart cardioblast phenotype of unc-5⁸

(Raza and Jacobs, 2016)

robo1¹/robo1[+] is a suppressor of embryonic heart cardioblast | embryonic stage phenotype of Dscam1¹

(Raza and Jacobs, 2016)

robo[+], Df(2R)BSC482, robo1¹, + is a suppressor of dopaminergic neuron phenotype of Trim9⁴⁶

(Song et al., 2011)

robo[+]/robo1¹ is a suppressor of larval somatic muscle cell phenotype of Lrt^UAS.EGFP, Scer\GAL4^sr-md710

(Wayburn and Volk, 2009)

robo1¹ is a suppressor of larval EW neuron phenotype of fra^unspecified

(Yang et al., 2009)

robo1¹ is a suppressor | partially of larval ventral nerve cord commissure | embryonic stage phenotype of Scer\GAL4^elav.PLu, fra^{ΔC.UAS.Tag:HA}, fra^unspecified

(Garbe et al., 2007)

robo[+]/robo1¹ is a suppressor of symmetrical commissure phenotype of elav⁵

(Simionato et al., 2007)

robo1¹ is a suppressor | partially of larval ventral nerve cord commissure phenotype of Scer\GAL4^elav.PLu, robo2^UAS.cSa

(Simpson et al., 2000)

robo1⁵/robo1¹ is a suppressor | partially of larval ventral nerve cord commissure phenotype of Scer\GAL4^elav.PLu, robo2^UAS.cSa

(Simpson et al., 2000)

NOT Suppressor of

Statement

Reference

robo1¹/robo1¹ is a non-suppressor of symmetrical commissure phenotype of Scer\GAL4^elav.PLu, Tcas\robo^{UAS.Tag:HA,Tag:SS(wg)}

(Evans and Bashaw, 2012)

robo1¹/robo1¹ is a non-suppressor of symmetrical commissure phenotype of Scer\GAL4^elav.PLu, Tcas\robo2-3^{UAS.Tag:HA,Tag:SS(wg)}

(Evans and Bashaw, 2012)

robo1¹ is a non-suppressor of larval longitudinal connective phenotype of Scer\GAL4^ap-md544, robo2^{UAS.Tag:HA,Tag:SS(wg)}

(Evans and Bashaw, 2010)

robo1¹/robo3¹ is a non-suppressor of larval longitudinal connective phenotype of Scer\GAL4^ap-md544, robo2^{UAS.Tag:HA,Tag:SS(wg)}

(Evans and Bashaw, 2010)

robo1¹/robo1¹ is a non-suppressor of larval anterior commissure phenotype of Scer\GAL4^elav.PLu, unc-5^{UAS.Tag:HA,Tag:SS(wg)}

(Keleman and Dickson, 2001)

robo1¹/robo1¹ is a non-suppressor of larval posterior commissure phenotype of Scer\GAL4^elav.PLu, unc-5^{UAS.Tag:HA,Tag:SS(wg)}

(Keleman and Dickson, 2001)

robo1⁸/robo1¹ is a non-suppressor of larval ventral nerve cord phenotype of Scer\GAL4^ap-md544, unc-5^{UAS.Tag:HA,Tag:SS(wg)}

(Keleman and Dickson, 2001)

robo1¹ is a non-suppressor of cephalopharyngeal skeleton phenotype of robo2^S4-14

(Schimmelpfeng et al., 2001)

Other

Statement

Reference

robo1¹, robo2^X123 has germinal proliferation center | embryonic stage 17 phenotype

(Anllo and DiNardo, 2022)

robo1¹, sli² has axon | embryonic stage phenotype

(Zang et al., 2022)

robo1¹, sli² has fascicle | embryonic stage phenotype

(Zang et al., 2022)

comm^Δe39, robo1¹, robo1^Tag:HA has embryonic midline of ventral nerve cord phenotype

(Daiber et al., 2021)

comm^Δe39, robo1¹, robo1^Tag:HA has commissure | embryonic stage phenotype

(Daiber et al., 2021)

Cele\sax-3^{10xUAS.Tag:HA,Tag:SS(wg)}, Scer\GAL4^elav.PLu, robo1¹ has embryonic midline of ventral nerve cord phenotype

(Daiber et al., 2021)

Cele\sax-3^robo1.Tag:HA, comm^Δe39, robo1¹ has embryonic midline of ventral nerve cord phenotype

(Daiber et al., 2021)

Cele\sax-3^robo1.Tag:HA, comm^Δe39, robo1¹ has commissure | embryonic stage phenotype

(Daiber et al., 2021)

Scer\GAL4^elav.PLu, robo1^{ΔFn1-3.Tag:HA}, robo1¹, robo2^{UAS.Tag:HA,Tag:SS(wg)} has axon | embryonic stage phenotype

(Brown and Evans, 2020)

Scer\GAL4^elav.PLu, robo1^{ΔFn1-3.Tag:HA}, robo1¹, robo2^{UAS.Tag:HA,Tag:SS(wg)} has embryonic/larval midline of ventral nerve cord | embryonic stage phenotype

(Brown and Evans, 2020)

Scer\GAL4^elav.PLu, robo1^ΔFn1.Tag:HA, robo1¹, robo2^{UAS.Tag:HA,Tag:SS(wg)} has axon | embryonic stage phenotype

(Brown and Evans, 2020)

Scer\GAL4^elav.PLu, robo1^ΔFn1.Tag:HA, robo1¹, robo2^{UAS.Tag:HA,Tag:SS(wg)} has embryonic/larval midline of ventral nerve cord | embryonic stage phenotype

(Brown and Evans, 2020)

Scer\GAL4^elav.PLu, robo1^ΔFn2.Tag:HA, robo1¹, robo2^{UAS.Tag:HA,Tag:SS(wg)} has axon | embryonic stage phenotype

(Brown and Evans, 2020)

Scer\GAL4^elav.PLu, robo1^ΔFn2.Tag:HA, robo1¹, robo2^{UAS.Tag:HA,Tag:SS(wg)} has embryonic/larval midline of ventral nerve cord | embryonic stage phenotype

(Brown and Evans, 2020)

Scer\GAL4^elav.PLu, robo1^ΔFn3.Tag:HA, robo1¹, robo2^{UAS.Tag:HA,Tag:SS(wg)} has axon | embryonic stage phenotype

(Brown and Evans, 2020)

Scer\GAL4^elav.PLu, robo1^ΔFn3.Tag:HA, robo1¹, robo2^{UAS.Tag:HA,Tag:SS(wg)} has embryonic/larval midline of ventral nerve cord | embryonic stage phenotype

(Brown and Evans, 2020)

Scer\GAL4^elav.PLu, robo1^{ΔIg2ΔFn3.Tag:HA}, robo1¹, robo2^{UAS.Tag:HA,Tag:SS(wg)} has axon | embryonic stage phenotype

(Brown and Evans, 2020)

Scer\GAL4^elav.PLu, robo1^{ΔIg2ΔFn3.Tag:HA}, robo1¹, robo2^{UAS.Tag:HA,Tag:SS(wg)} has embryonic/larval midline of ventral nerve cord | embryonic stage phenotype

(Brown and Evans, 2020)

Scer\GAL4^elav.PLu, robo1^{ΔIg2-5.Tag:HA}, robo1¹, robo2^{UAS.Tag:HA,Tag:SS(wg)} has axon | embryonic stage phenotype

(Brown and Evans, 2020)

Scer\GAL4^elav.PLu, robo1^{ΔIg2-5.Tag:HA}, robo1¹, robo2^{UAS.Tag:HA,Tag:SS(wg)} has embryonic/larval midline of ventral nerve cord | embryonic stage phenotype

(Brown and Evans, 2020)

Scer\GAL4^elav.PLu, robo1¹, robo1^Tag:HA, robo2^{UAS.Tag:HA,Tag:SS(wg)} has axon | embryonic stage phenotype

(Brown and Evans, 2020)

Scer\GAL4^elav.PLu, robo1¹, robo1^Tag:HA, robo2^{UAS.Tag:HA,Tag:SS(wg)} has embryonic/larval midline of ventral nerve cord | embryonic stage phenotype

(Brown and Evans, 2020)

robo1¹/robo1[+], sli² has medial longitudinal fascicle | ectopic phenotype

(Garbe et al., 2007)

robo1¹, sli² has larval longitudinal connective | embryonic stage 16 phenotype

(Keleman et al., 2005)

robo1¹, sli² has commissure | embryonic stage 16 phenotype

(Keleman et al., 2005)

robo1¹, robo2^x135 has mesothoracic dorsal triscolopidial chordotonal organ dch3 phenotype

(Kraut and Zinn, 2004)

robo1¹, robo2^x135 has metathoracic dorsal triscolopidial chordotonal organ dch3 phenotype

(Kraut and Zinn, 2004)

robo1¹, lea[+]/robo2⁸ has larval neuron phenotype

(Lee et al., 2004)

chb⁴, robo1¹ has larval neuron phenotype

(Lee et al., 2004)

chb⁴/chb[+], robo1¹ has larval neuron phenotype

(Lee et al., 2004)

robo1¹, robo2⁸ has larval neuron phenotype

(Lee et al., 2004)

robo1¹, robo3¹ has larval abdominal ventral cluster group multidendritic neuron phenotype

(Orgogozo et al., 2004)

robo1¹, lea[+]/robo2⁸ has presumptive embryonic/larval central nervous system phenotype

(Wills et al., 2002)

robo1¹, robo3[+]/robo3¹ has presumptive embryonic/larval central nervous system phenotype

(Wills et al., 2002)

robo1¹, robo2^X123 has muscle cell of A1-7 ventral longitudinal muscle 3 phenotype

(Kramer et al., 2001, Kramer et al., 2001)

robo1¹, robo2^X123 has muscle cell of A1-7 ventral longitudinal muscle 4 phenotype

(Kramer et al., 2001, Kramer et al., 2001)

Ptp10D¹, Ptp69D¹/Ptp69D^8ex25, robo1¹ has larval longitudinal connective phenotype

(Sun et al., 2000)

robo1¹, sli¹ has presumptive embryonic/larval central nervous system phenotype

(Kidd et al., 1999)

robo1¹, sli² has presumptive embryonic/larval central nervous system phenotype

(Kidd et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

In robo1¹, robo2^X123 double mutant stage 17 embryos, the testes often exhibit dispersed aggregates of germline niche cells, unlike the single anterior niche position in controls.

(Anllo and DiNardo, 2022)

robo1¹, robo2^lea-2 double homozygous embryonic heart cardioblast show similar cell rounding, slow migration and decreased filopodial and lamellopodial activities) to those observed in robo1¹ single homozygotes; these defects are associated with gaps in the leading edge (and lost adhesions with ipsilateral partners), cell clumping, improper linear alignment of the cardioblasts, and heart lumen formation defects, as compared to controls. The decreased lamellopodial activity, but not the decreased filopodial activity, slow migration, or heart lumen formation, are only partially suppressed by the expression of robo1^Scer\UAS.cKa under the control of Scer\GAL4^Mef2.247.

Only the reduced filopodial and lamellopodial activities observed in robo1¹ homozygous embryonic heart cardioblasts, but not their reduced migration velocity, nor the associated heart lumen formation defects, are partially suppressed by the expression of fra^Scer\UAS.cKa under the control of Scer\GAL4^Mef2.247.

sli², robo1¹ and fra³, robo1¹ double heterozygotes show a more severe decrease in filopodial activity compared to either single heterozygote conditions and a more severe decrease in lamellopodial activity compared to robo1¹ heterozygotes, but do not show significant changes in migration velocity compared to controls. unc-5⁸, robo1¹ double heterozygotes show a less severe decrease in migration velocity compared to either single heterozygote conditions, a more severe decrease in filopodial activity compared to either single heterozygote conditions, and a more severe decrease in lamellopodial activity compared to robo1¹ heterozygotes. Dscam1¹, robo1¹ double heterozygotes show a more severe decrease in filopodial activity compared to either single heterozygote conditions, a more severe decrease in lamellopodial activity compared to robo1¹ heterozygotes, and no migration velocity defects compared to wild-type controls. Dscam1⁰⁵⁵¹⁸, robo1¹ double heterozygotes show a similar decrease in filopodial and lamellopodial activities compared to either single heterozygote conditions, and show no significant changes in migration velocity compared to wild-type controls.

The decreased filopodial and lamellopodial activities, but not the decreased migration velocity, observed in Dscam1¹/Dscam1⁰⁵⁵¹⁸ transheterozygous embryonic heart cardioblasts are enhanced by robo1¹ heterozygosity.

(Raza and Jacobs, 2016)

A robo¹ homozygous background increases the percentage of embryonic segments displaying eg-positive axon midline crossing defects in flies expressing fra^{ΔC.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^eg-Mz360. However, this increase isn't considered statistically significant.

(O'Donnell and Bashaw, 2013)

Removal of one copy of robo¹ and sli (using the deficiency Df(2R)BSC482) suppresses the axon midline crossing defect found in Trim9⁴⁶ mutants.

(Song et al., 2011)

A robo¹ mutant genetic background does not affect the ability of ectopic Scer\GAL4^ap-md544>lea^{Scer\UAS.T:Ivir\HA1,T:SS-wg} to direct the Scer\GAL4^ap-md544-expressing axons to lateral pathways.

In robo3¹, robo¹ double mutants, the intermediate Fas2-positive tract fuses with the medial tract, and this thick fascicle crosses the midline. As in robo¹ mutants, the Scer\GAL4^ap-md544-expressing axons follow this fascicle across the midline. The lateral Fas2-positive tract remains in robo3¹, robo¹ double mutants.

Ectopic Scer\GAL4^ap-md544>lea^{Scer\UAS.T:Ivir\HA1,T:SS-wg} is able to direct the Scer\GAL4^ap-md544-expressing axons to extreme lateral positions even in a robo3¹, robo¹ mutant genetic background.

(Evans and Bashaw, 2010)

The midline crossing errors seen in Fas2-expressing longitudinal axons of robo¹ homozygous embryos are almost fully rescued if they are carrying one of robo::robo3^{1.CC2.3.CC3.robo.T:Ivir\HA1} or robo::robo3^{3.Ig2.1.Ig3.robo.T:Ivir\HA1}.

The midline crossing errors seen in Fas2-expressing longitudinal axons of robo¹ homozygous embryos are not rescued if they are carrying one of lea^{robo.T:Ivir\HA1}, robo3^{robo.T:Ivir\HA1}, robo::robo3^{1.Ig2.3.Ig3.robo.T:Ivir\HA1}, robo::robo3^{1.TM.3.CC0.robo.T:Ivir\HA1}, robo::robo3^{1.CC0.3.CC1.robo.T:Ivir\HA1}, robo::robo3^{1.CC1.3.CC2.robo.T:Ivir\HA1} or robo::robo3^{3.CC2.1.CC3.robo.T:Ivir\HA1}.

The midline crossing errors seen in Fas2-expressing longitudinal axons of robo¹ homozygous embryos are partially rescued if they are carrying one of robo::robo3^{3.TM.1.CC0.robo.T:Ivir\HA1}, robo::robo3^{3.CC0.1.CC1.robo.T:Ivir\HA1} or robo::robo3^{3.CC1.1.CC2.robo.T:Ivir\HA1}.

(Spitzweck et al., 2010)

robo¹/+ completely rescues the abnormal muscle phenotype caused by expression of Lrt^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^sr-md710.

(Wayburn and Volk, 2009)

The EW neuron guidance defects in fra^unspecified robo¹ double mutants are less severe than fra^unspecified single mutants.

(Yang et al., 2009)

Many of the dorsal abdominal clusters in robo¹/lea^robo2-8 mutant animals have branches that exceed the normal level of extension at approximately 21 and 22 hours after egg laying, but this phenotype is not significantly greater than the defects observed in robo¹ mutants.

(Dimitrova et al., 2008)

Expression of fra^{Scer\UAS.T:Ivir\HA1.cGa} under the control of Scer\GAL4^insc-Mz1407 does not suppress the ectopic commissural crossing phenotype seen in homozygous robo¹ mutant embryos.

Expression of fra::robo^{SD.Scer\UAS.T:SV5\V5,T:Zzzz\His6,T:Ivir\HA1,T:SS-wg} under the control of Scer\GAL4^insc-Mz1407 almost completely rescues the ectopic commissural crossing phenotype seen in homozygous robo¹ mutant embryos. Ectopic crossings are only seen in 20% of segments.

(Gilestro, 2008)

sli², robo¹/+ embryos show midline axon guidance defects, with on average two to three medial longitudinal fascicle ectopically crossing the midline per embryo.

(Garbe et al., 2007)

Expression of fra^{ΔC.Scer\UAS.T:Ivir\HA} driven by Scer\GAL4^elav.PLu in a fra^unspecified robo¹ double mutant background dramatically repels axons, though the defect is less severe than seen in a fra^unspecified single mutant background.

Expression of fra^{ΔC.Scer\UAS.T:Ivir\HA} driven by Scer\GAL4^elav.PLu leads to a partial suppression of the robo¹ single mutant neuronal phenotype.

(Garbe et al., 2007)

Reduction of robo in a robo¹ heterozygous background partially suppresses the elav⁵ mutant commissural phenotype. Thicker commissural tracts (relative to elav⁵ embryos) are made in more than 50% of the neuromeres.

(Simionato et al., 2007)

Ts(1Lt;YSt)B118+Ts(1Rt;YLt)T9/Y partially suppresses the dMP2 axon misprojection phenotype seen in homozygous robo¹ embryos, reducing the penetrance of the contralateral misprojection along the anterior commissure of the next segment from 99% to 61%.

fra³ strongly suppresses the dMP2 axon misprojection phenotype seen in homozygous robo¹ embryos, reducing the penetrance of the contralateral misprojection along the anterior commissure of the next segment from 99% to 24%. Expression of fra^{ΔC.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^insc-Mz1407, but not under the control of Scer\GAL4^15J2, restores the aberrant midline-crossing phenotype in these animals.

The pCC and vMP2 midline-crossing phenotypes seen in homozygous robo¹ embryos are partially and strongly suppressed by Ts(1Lt;YSt)B118+Ts(1Rt;YLt)T9 respectively.

The pCC and vMP2 midline-crossing phenotypes seen in homozygous robo¹ embryos are partially and strongly suppressed by fra³ respectively. Expression of fra^{ΔC.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4⁶⁰⁵ restores the aberrant midline-crossing phenotype in these animals.

23.8% of dMP2 neurons project contralaterally in sli^GA20/robo¹ transheterozygous embryos. This phenotype is suppressed by expression of sli^Scer\UAS.cKa under the control of Scer\GAL4⁶⁰⁵.

(Hiramoto and Hiromi, 2006)

In robo¹,sli² /+,+ stage 16 embryos, FasII axons cross the midline in three to five segments per embryo.

Expression of Nedd4^{Scer\UAS.T:Hsap\MYC} under the control of either Scer\GAL4^elav.PLu or Scer\GAL4¹⁴⁰⁷ does not enhance the midline crossing phenotype seen in transheterozygous sli² robo¹ stage 16 embryos.

Expression of Nedd4^EY00500 under the control of either Scer\GAL4^elav.PLu or Scer\GAL4¹⁴⁰⁷ does not enhance the midline crossing phenotype seen in transheterozygous sli² robo¹ stage 16 embryos.

Df(3R)ED4688 does not enhance the midline crossing phenotype seen in transheterozygous sli² robo¹ stage 16 embryos.

Expression of ena^Scer\UAS.cCa under the control of Scer\GAL4^elav.PLu suppresses the midline crossing phenotype seen in transheterozygous sli² robo¹ stage 16 embryos.

(Keleman et al., 2005)

In robo¹ lea^robo2-1 double mutants, the salivary gland guidance phenotype is enhanced. Glands are curved medially towards the midline, instead of lying parallel to the midline as in wild type.

(Kolesnikov and Beckendorf, 2005)

robo¹, lea^robo2-8 double mutants show severe myocardial cell misalignment including gaps, intercalation, and double rows.

(Qian et al., 2005)

35.9% of thoracic segments show transformation of the dch3 chordotonal organs to a morphology resembling that of abdominal lch5 chordotonal organs in robo¹ lea^x135 double mutant embryos.

(Kraut and Zinn, 2004)

robo¹ chb⁴ double heterozygous embryos have axons ectopically crossing the midline. Heterozygosity for lea^robo2-8 enhances the frequency of axons ectopically crossing the midline that is seen in robo¹ chb⁴ double heterozygous embryos. Heterozygosity for robo3¹ does not enhance the frequency of axons ectopically crossing the midline that is seen in robo¹ chb⁴ double heterozygous embryos. robo¹ lea^robo2-8 double heterozygous embryos have axons ectopically crossing the midline.

(Lee et al., 2004)

robo¹ robo3¹ double mutant embryos do not show defects in the position of the vmd1a neuron of the ventral multidendritic neuron cluster in the abdomen; a single vmd1a neuron is seen in each hemisegment. robo¹ lea^X123 double mutant embryos show defects in the position of the vmd1a neuron of the ventral multidendritic neuron cluster in the abdomen; in 21% of segments loss of the vmd1a neuron in a hemisegment is associated with duplication of the vmd1a neuron in the contralateral hemisegment. In addition, in 36% of segments, some vmd1a neurons are missing from the ventral vmd clusters (in most of these segments, the missing md neurons are probably located near the midline).

(Orgogozo et al., 2004)

A few axon bundles cross the midline incorrectly in embryos trans-heterozygous for robo¹ and sli¹. This combination increases the penetrance of crossovers to about 60%.

When one copy of Abl⁴ is also present in trans-heterozygous robo¹ and sli¹ embryos, several abnormal crossovers are observed in all embryos examined, and some axon bundles collapse towards the midline in a manner reminiscent of a sli¹ phenotype.

Heterozygous robo¹ enhances the number of abnormal axon crossovers observed in Abl⁴ mutants.

The addition of one mutant copy of Abl⁴ doubles the number of crossovers observed in heterozygous robo¹ mutants. The presence of two copies of Abl⁴ in heterozygous robo¹ mutants increases the penetrance of crossovers to 90%, with most embryos exhibiting three or more crossovers.

In double homozygous robo¹; Abl⁴ embryos, several axon bundles cross the midline abnormally as gaps and thinning of the longitudinal connectives become evident.

Compared with heterozygous robo¹ mutants alone, overexpression of Abl^Scer\UAS.cHa using Scer\GAL4^ftz.ng enhances the frequency of abnormal axonal crossovers.

Compared with 26% of heterozygous robo¹ mutants exhibiting inappropriate crossovers, expression of Abl^KN.Scer\UAS in the Scer\GAL4^ftz.ng pattern results in almost all (95%) embryos exhibiting several axon bundles crossing the midline.

(Hsouna et al., 2003)

robo¹ lea^robo2-8 double mutant embryos show misprojection of lch5 axons to the v'ch1 axon pathway in 11% of hemisegments (a similar frequency to robo¹ single mutants). Stalling of the lch5 axons prior to turning point TP2, a characteristic of the lea^robo2-8 single mutant, is also seen in double mutant embryos, although at a higher frequency (6% of hemisegments). The dorsal cluster misprojection phenotype seen in lea^robo2-8 single mutant embryos is not seen in the robo¹ lea^robo2-8 double mutant embryos; in the double mutant embryos the dorsal cluster axons follow a normal trajectory to the lateral region, although in 7% of hemisegments they grow abnormally from this point, either projecting along the v'ch1 route or growing intersegmentally. The lch5 cell bodies are located dorsal to their normal position in 11% of hemisegments and are aberrantly oriented in 3% of hemisegments of robo¹ lea^robo2-8 double mutant embryos. Missing or aberrantly positioned spiracular branches and failure of motor axons to extend as far as the sensory neuron cluster are occasionally seen.

(Parsons et al., 2003)

The addition of Rho1^N19.Scer\UAS (driven by Scer\GAL4^ftz.ng) to robo¹/+ embryos enhances the midline crossover phenotype seen in the pCC/MP2 pathway axons. 89% of embryos exhibit the phenotype. An average of 3.1 crossovers are seen per embryo. The addition of Rho1^V14.Scer\UAS (driven by Scer\GAL4^ftz.ng) to robo¹/+ embryos enhances the midline crossover phenotype seen in the pCC/MP2 pathway axons. 48% of embryos exhibit the phenotype. An average of 2.0 crossovers are seen per embryo. The addition of Rac1^N17.Scer\UAS (driven by Scer\GAL4^ftz.ng) to robo¹/+ embryos has no effect on the midline crossover phenotype seen in the pCC/MP2 pathway axons. The addition of Rac1^V12.Scer\UAS (driven by Scer\GAL4^ftz.ng) to robo¹/+ embryos enhances the midline crossover phenotype seen in the pCC/MP2 pathway axons. 100% of embryos exhibit the phenotype. An average of 6.5 crossovers are seen per embryo. The addition of Cdc42^N17.Scer\UAS (driven by Scer\GAL4^ftz.ng) to robo¹/+ embryos partially suppresses the midline crossover phenotype seen in the pCC/MP2 pathway axons. 18% of embryos exhibit the phenotype. An average of 1.2 crossovers are seen per embryo. The addition of Cdc42^V12.Scer\UAS (driven by Scer\GAL4^ftz.ng) to robo¹/+ embryos has no effect on the midline crossover phenotype seen in the pCC/MP2 pathway axons.

(Fritz and VanBerkum, 2002)

robo¹/+ heterozygous embryos, in which expression of Gα49B^{Q203L.Scer\UAS} is regulated under the control of Scer\GAL4^ftz.ng, exhibit a significant increase in the number of midline crossovers (of axons expressing Fas2), as compared to robo¹/+ mutant embryos and Gα49B^{Q203L.Scer\UAS};Scer\GAL4^ftz.ng embryos (316/623 abdominal segments exhibit midline crossing in the combined mutant compared to 12/245 in robo¹ and 86/427 in Gα49B^{Q203L.Scer\UAS};Scer\GAL4^ftz.ng).

(Ratnaparkhi et al., 2002)

One copy of capt^k01217 enhances the frequency of midline crossing defects seen in robo¹ lea^robo2-8 double mutant heterozygotes. One copy of capt¹⁰ does not enhance the frequency of midline crossing defects seen in robo¹ robo3¹ double mutant heterozygotes.

(Wills et al., 2002)

The abnormal midline crossing of axons seen in robo¹ heterozygotes is suppressed if the embryos are also heterozygous for one of chic^sand-1, chic^gdh-5 or chic²²¹. However, chic^sand-1 robo¹ double homozygotes show a severe disruption of the nerve cord. Large numbers of axons cross the midline within segment boundaries and major gaps are seen in the longitudinal connectives.

(Kim et al., 2001)

87% of segments show crossing of the midline by muscles 6/7 in robo¹ lea^X123 double mutant embryos. This defect is rescued by expression of robo^Scer\UAS.cKa under the control of Scer\GAL4^how-24B, but the axonal phenotype of these embryos is mutant. 6% of segments show crossing of the midline by muscles 6/7, 8% show abnormal insertion of muscle 5 and 11% show abnormal insertion of muscles 6/7 in sli² robo¹ double mutant embryos. 15% of segments show crossing of the midline by muscles 6/7, 31% show abnormal insertion of muscle 5 and 16% show abnormal insertion of muscles 6/7 in sli² robo¹ lea^X123 triple mutant embryos.

(Kramer et al., 2001)

Muscles 6 and 7 cross the midline in most segments in robo¹ lea^X123 double mutant embryos.

(Kramer et al., 2001)

robo¹ lea^S4-14 double mutant embryos have a head phenotype comparable to that of lea^S4-14 single mutants.

(Schimmelpfeng et al., 2001)

Sos^JC2 suppresses the axon crossing over phenotype seen in robo¹ heterozygotes.

(Fritz and VanBerkum, 2000)

robo¹, lea^robo2-5 embryos show an enhancement of the embryonic nervous system phenotype seen in these embryos. The pCC axon fasciculates with its contralateral homologue to extend anteriorly right along the midline. The aCC axons also frequently cross the midline in these embryos. By stage 16, all axons have joined the single midline bundle. Double mutants also exhibit a embryonic muscle phenotype. Ventral muscles normally attach to the epidermis beneath the lateral central nervous system (CNS). In robo¹/lea^robo2-5 mutants, these muscles frequently extend across the dorsal surface of the CNS. Some muscles still attach to beneath the CNS though often somewhat close to the midline than usual. The addition of comm¹ to robo¹, lea^robo2-5 embryos has no effect on the ventral nerve cord phenotype. robo¹/robo3¹ show an embryonic nervous system that is merely additive.

(Rajagopalan et al., 2000)

Ptp10D¹; robo¹/robo¹; Ptp69D¹/Ptp69D^8ex25 triple mutant embryos have a severe phenotype in which most Fas2-positive axons converge on the midline. Some circles around the midline are still seen, but many axons fasciculate into a single thick bundle that extends along the midline. Vestiges of the lateral longitudinal pathways are seen in some segments.

(Sun et al., 2000)

Embryos heterozygous for both robo¹ and sli² show deviation of longitudinal fascicles towards the midline in 74% of segments. Embryos heterozygous for both robo¹ and sli^E-158 show deviation of longitudinal fascicles towards the midline in 32% of segments.

(Battye et al., 1999)

28% of segments contain Fas2-positive neurons inappropriately crossing the midline in sli¹/robo¹ double heterozygous embryos, in contrast to either single heterozygote which do not show this defect. 36% of segments contain Fas2-positive neurons inappropriately crossing the midline in sli²/robo¹ double heterozygous embryos, in contrast to either single heterozygote which show defects in 1 or 0% of segments.

(Kidd et al., 1999)

Xenogenetic Interactions

Statement

Reference

The ectopic midline crossing phenotype seen in the medial Fas2-positive axons in robo1¹ embryos is suppressed if they are expressing either Tcas\robo^{Scer\UAS.T:Ivir\HA1,T:SS-wg} or Tcas\robo2-3^{Scer\UAS.T:Ivir\HA1,T:SS-wg} under the control of Scer\GAL4^elav.PLu, with nearly all segments being commissureless in these animals.

(Evans and Bashaw, 2012)

Expression of Mmus\Itpr1^{sponge.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^15J2 suppresses the dMP2 axon misprojection phenotype seen in homozygous robo¹ embryos in 30% of axons.

Expression of Mmus\Itpr1^{mut.sponge.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^15J2 does not suppress the dMP2 axon misprojection phenotype seen in homozygous robo¹ embryos.

(Hiramoto and Hiromi, 2006)

Compared with heterozygous robo¹ mutants alone, expression of Abl::Hsap\ABL1::Hsap\BCR^{P210.Scer\UAS} using Scer\GAL4^ftz.ng enhances the frequency of abnormal axonal crossovers. Almost every segment of every robo¹/+ embryo expressing Abl::Hsap\ABL1::Hsap\BCR^{P210.Scer\UAS} exhibits abnormal crossovers.

Expression of Abl::Hsap\ABL1::Hsap\BCR^KR.Scer\UAS using Scer\GAL4^ftz.ng enhances the midline-crossing phenotype of axon bundles in heterozygous robo¹ embryos.

(Hsouna et al., 2003)

Fas2-positive axon bundles cross the midline in about 83% or 88% of robo¹/+ embryos which are also expressing Ggal\MLCK^ct.Scer\UAS under the control of Scer\GAL4^ftz.ng.

(Kim et al., 2002)

The number of embryos displaying abnormal midline crossing of axons increases from 5% in Khc::Ggal\MLCK^KA.ftz heterozygotes to just over 60% if the embryos are also heterozygous for robo¹.

(Kim et al., 2001)

The penetrance of the Fas2-positive axon crossover phenotype is increased in robo¹ embryos that also carry Khc::Ggal\MLCK^KA.ftz compared to either single mutant alone. A loss of integrity of the longitudinal connectives is seen in the robo¹ Khc::Ggal\MLCK^KA.ftz double mutant embryos and only remnants of the pCC/MP2 pathway are evident. The pioneer neurons of the pCC/MP2 and MP1 pathways cross the midline as they reach the anterior commissure in homozygous embryos. Axons of the MP1 cluster are also seen to cross the midline directly, join their sister neurons on the contralateral side and recross the midline at the anterior commissure. In embryos heterozygous for robo¹ and carrying two copies of Khc::Ggal\MLCK^KA.ftz, pioneer neurons of the pCC/MP2 and MP1 pathways will periodically cross the midline. Khc::Ggal\MLCK^KA.ftz mediated stalls are still evident, but in addition, the pCC neuron will occasionally cross the midline and pioneer neurons within the MP cluster extend axons across the midline as observed in robo mutants. Sos^JC2 partially suppresses the interaction between robo¹ and Khc::Ggal\MLCK^KA.ftz.

(Fritz and VanBerkum, 2000)

Complementation and Rescue Data

Rescued by

robo1¹ is rescued by robo1^Tag:HA

(Loy et al., 2025)

robo1¹ is rescued by robo1^{10xUAS.Tag:HA,Tag:SS(wg)}/Scer\GAL4^elav.PLu

(Daiber et al., 2021)

robo1¹ is rescued by robo1^Tag:HA

(Daiber et al., 2021)

robo1¹ is rescued by robo1^{ΔFn1-3.Tag:HA}

(Brown and Evans, 2020)

robo1¹ is rescued by robo1^{ΔIg2ΔFn2.Tag:HA}

(Brown and Evans, 2020)

robo1¹ is rescued by robo1^{ΔIg2-4ΔFn1-3.Tag:HA}

(Brown and Evans, 2020)

robo1¹ is rescued by robo1^{ΔIg2-5.Tag:HA}

(Brown and Evans, 2020)

robo1¹ is rescued by robo1^{ΔIg3ΔFn3.Tag:HA}

(Brown and Evans, 2020)

robo1¹ is rescued by robo1^Tag:HA

(Brown and Evans, 2020)

robo1¹ is rescued by robo1^ΔIg3.Tag:HA

(Reichert et al., 2016)

robo1¹ is rescued by robo1^ΔIg4.Tag:HA

(Reichert et al., 2016)

robo1¹ is rescued by robo1^ΔIg5.Tag:HA

(Reichert et al., 2016)

robo1¹ is rescued by robo1^Tag:HA

(Reichert et al., 2016)

robo1¹ is rescued by robo1^Tag:HA

(Brown et al., 2015)

robo1¹ is rescued by robo1^{10xUAS.Tag:HA,Tag:SS(wg)}/Scer\GAL4^elav.PLu

(Evans and Bashaw, 2012)

robo1¹ is rescued by robo1^robo1.Tag:HA

(Spitzweck et al., 2010)

robo1²/robo1¹ is rescued by robo1^UAS.cKa/Scer\GAL4^eve.CQ2

(Mauss et al., 2009)

robo1¹ is rescued by Scer\GAL4^insc-Mz1407/robo1^{UAS.Tag:V5,Tag:polyHis,Tag:HA,Tag:SS(wg)}

(Gilestro, 2008)

robo1¹ is rescued by robo1^UAS.Tag:MYC/Scer\GAL4^ap-md544

(Garbe et al., 2007)

robo1¹ is rescued by robo1^+t16

(Kidd et al., 1998)

Partially rescued by

robo1¹ is partially rescued by robo1^{10xUAS.Tag:HA,Tag:SS(wg)}/Scer\GAL4^elav.PLu

(Loy et al., 2025)

robo1¹ is partially rescued by robo1^{ΔIg2ΔFn3.Tag:HA}

(Brown and Evans, 2020)

robo1¹ is partially rescued by Scer\GAL4^Mef2.247/robo1^UAS.cKa

(Raza and Jacobs, 2016)

robo1¹ is partially rescued by robo1^ΔIg2.Tag:HA

(Reichert et al., 2016)

robo1¹ is partially rescued by robo1^{10xUAS.Tag:HA,Tag:SS(wg)}/Scer\GAL4^elav.PLu

(Brown et al., 2015)

robo1¹ is partially rescued by robo1^{UAS.Tag:HA,Tag:SS(wg)}/Scer\GAL4^109(2)80

(Dimitrova et al., 2008)

robo1¹ is partially rescued by robo1^UAS.Tag:MYC/Scer\GAL4^15J2

(Hiramoto and Hiromi, 2006)

Not rescued by

robo1¹ is not rescued by robo1^ΔIg1.Tag:HA

(Reichert et al., 2016)

robo1¹ is not rescued by robo1^{ΔIg1.UAS.Tag:HA,Tag:SS(wg)}/Scer\GAL4^elav.PLu

(Brown et al., 2015)

robo1¹ is not rescued by robo1^ΔIg1.Tag:HA

(Brown et al., 2015)

Comments

The robo1¹ homozygous embryonic heart cardioblast defects (i.e. migration velocity and in filopodial and lamellopodial extensions and activities) and embryonic heart lumen formation defects are partially rescued by the expression of robo1^Scer\UAS.cKa under the control of Scer\GAL4^Mef2.247.

(Raza and Jacobs, 2016)

The defects in midline repulsion in the ventral nerve cord reflected by the ectopic midline axon crossings characteristic for robo1¹ homozygous mutant embryos is fully rescued by combination with any of the following: robo1^T:Ivir\HA1, robo1^{ΔIg3.T:Ivir\HA1}, robo1^{ΔIg4.T:Ivir\HA1} or robo1^{ΔIg5.T:Ivir\HA1}, is partially rescued by expression of robo1^{ΔIg2.T:Ivir\HA1} and not affected by expression of robo1^{ΔIg1.T:Ivir\HA1} in the mutant background.

(Reichert et al., 2016)

Expression of robo1^{10xScer\UAS.T:Ivir\HA1,T:SS-wg} under the control of Scer\GAL4^elav.PLu rescues the FasII-positive axon guidance defects of robo1¹/robo1¹ mutants, but also causes additional axon guidance defects, including disruption of normal commissure formation and disorganization of longitudinal axon pathways.

Expression of robo1^{ΔIg1.Scer\UAS.T:Ivir\HA1,T:SS-wg} under the control of Scer\GAL4^elav.PLu does not rescue the FasII-positive axon guidance defects of robo1¹/robo1¹ mutants.

Expression of two copies of robo1^T:Ivir\HA1, but not robo1^{ΔIg1.T:Ivir\HA1}, rescues the ventral nerve cord defects, FasII-expressing axon guidance defects and pCC guidance defects of robo1¹/robo1¹ mutant embryos.

(Brown et al., 2015)

Expression of robo1^{10xScer\UAS.T:Ivir\HA1,T:SS-wg} under the control of Scer\GAL4^elav.PLu rescues the ectopic midline crossing phenotype seen in the medial Fas2-positive axons in robo1¹ embryos.

(Evans and Bashaw, 2012)

robo^{robo.T:Ivir\HA1} almost fully rescues the midline crossing errors seen in Fas2-expressing longitudinal axons of robo¹ homozygous embryos.

(Spitzweck et al., 2010)

The ectopic midline innervation phenotype found in robo¹/robo² mutants can be rescued by expression of robo^Scer\UAS.cKa under the control of Scer\GAL4^eve.CQ2.

(Mauss et al., 2009)

Expression of robo^{Scer\UAS.T:Ivir\HA1,T:wg} under the control of Scer\GAL4^109(2)80 in robo¹ mutant animals partially rescues the mutant dendritic overgrowth phenotype from 58% to 18%.

(Dimitrova et al., 2008)

Expression of robo^{Scer\UAS.T:SV5\V5,T:Zzzz\His6,T:Ivir\HA1,T:SS-wg} under the control of Scer\GAL4^insc-Mz1407 almost completely rescues the ectopic commissural crossing phenotype seen in homozygous robo¹ mutant embryos.

(Gilestro, 2008)

Expression of robo^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^15J2 rescues the contralateral misprojection phenotype of dMP2 axons seen in robo¹ embryos, while many surrounding neurons misproject contralaterally.

(Hiramoto and Hiromi, 2006)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8755

y¹ w^*; robo1¹/CyO, P{ase-lacZF:2.0}PK2

Notes on Origin

Discoverer

Induced on: Fas3 null chromosome.

(Kidd et al., 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

GA285

(Yasunaga et al., 2015, Simpson et al., 2000, Kidd et al., 1998)

Robo^GA285

(Johnson et al., 2004)

robo-1^GA285

(Hernandez-Fleming et al., 2017)

robo1¹

(Loy et al., 2025, de Torres-Jurado et al., 2022, Huang et al., 2022, Daiber et al., 2021, Brown and Evans, 2020, Brown et al., 2018, Reichert et al., 2016, Brown et al., 2015, Spitzweck et al., 2010, Battye et al., 2001)

robo1^GA285

(Anllo and DiNardo, 2022, Sato et al., 2019, Simionato et al., 2007, Kolesnikov and Beckendorf, 2005)

robo^GA2875

(Brierley et al., 2009)

robo^GP285

(Kraut and Zinn, 2004)

robo^Ga285

(Garbe et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (86)

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