FlyBase Allele Report: Dmel\grk[2B6]

General Information

Symbol

Dmel\grk^2B6

Species

D. melanogaster

Name

FlyBase ID

FBal0031404

Feature type

allele

Associated gene

Dmel\grk

Associated Insertion(s)

Carried in Construct

Also Known As

grk^2B

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Neuman-Silberberg and Schupbach, 1996, Neuman-Silberberg and Schupbach, 1993)

Progenitor genotype

Cytology

Description

442bp deletion starting at nucleotide 236.

(Thio et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:8,433,451..8,433,896 [-]

Comment:

A 442 bp deletion is reported in FBrf0127360; due to differences between the reference sequence and the sequence reported in GB:AF223394, the endpoints of the 442 bp deletion in GB:AF223394 map to a slightly longer fragment in the reference sequence

(Thio et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile | recessive

(Neuman-Silberberg and Schupbach, 1993)

Phenotype Manifest In

border follicle cell (with grk¹)

(Duchek and Rorth, 2001)

dorsal appendage (with grk^2E12)

(Niepielko and Yakoby, 2014)

dorsal appendage | cold sensitive

(Doronkin et al., 2002)

dorsal appendage | embryonic stage 12 (with grk³)

(Atkey et al., 2006)

egg chorion (with grk^2E12)

(Niepielko and Yakoby, 2014)

egg operculum (with grk^HF)

(Shravage et al., 2007)

embryo

(Queenan et al., 1999)

microtubule & oocyte

(Clegg et al., 1997)

oocyte & microtubule (with grk¹)

(Januschke et al., 2002)

oocyte | germline clone

(Caceres and Nilson, 2005)

ovary (with grk¹)

(Januschke et al., 2002, Duchek and Rorth, 2001)

Detailed Description

Statement

Reference

Eggs derived from grk^2B6/grk^HF females lack operculum regions II and III (the section that normally extends from the area surrounding the micropyle to the dorsal appendages and the section that is normally between the dorsal appendages), and instead only region I cells are present symmetrically surrounding the micropyle, following a symmetric collar that separates the operculum from the rest of the chorion.

(Shravage et al., 2007)

Stage 12 grk³/grk^2B6 mutant egg chambers lack dorsal appendages.

(Atkey et al., 2006)

In egg chambers with grk^2B6 germline clones, the oocyte nucleus is incorrectly located at the posterior of the oocyte in 94/154 cases observed. In egg chambers with a mosaic germline composed of eight grk^2B6 nurse cells, seven wild-type nurse cells and a wild-type oocyte, the oocyte nucleus is properly localized at the anterior margin of the oocyte in all observed cases. Likewise, the oocyte is also anteriorly localized when egg chambers have a grk^2B6 oocyte with eight grk^2B6 nurse cells and seven wild-type nurse cells. In germline mosaics with a grk^2B6 oocyte, the border cell cluster is correctly dorsally localized and dorsal follicle cell fates are not affected.

(Caceres and Nilson, 2005)

Heterozygous females lay eggs with fused or partially fused dorsal appendages (75% at 18^oC, 27.7% at 25^oC and 13.8% at 29^oC).

(Doronkin et al., 2002)

In grk^2B6/grk¹ oocytes the anterior-posterior gradient of microtubule concentration seen in wild-type is abolished. However, concentration of microtubules around the mis-localized nucleus persists.

(Januschke et al., 2002)

Ovaries from grk¹/grk^2B6 mutant females show a range of defects. In mildly affected egg chambers where the germinal vesicle has moved anterior and dorsal, border cells complete posterior migration but fail to migrate dorsally.

(Duchek and Rorth, 2001)

Embryos laid by homozygous grk^2B6 mothers are strongly ventralised.

(Queenan et al., 1999)

Microtubule cytoskeleton in grk^2B6/grk¹ oocytes is defective due to premature streaming of the microtubules.

(Clegg et al., 1997)

grk^2B6/grk^2E12 egg chambers show a duplication of all three anterior follicle cell types at the posterior pole.

(Gonzalez-Reyes et al., 1995)

grk^2B6/grk² heterozygous eggs lack dorsal ventral polarity and possess a posterior micropyle. The eggs never contain embryos.

(Roth et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

grk³/grk^2B6 is a suppressor of increased cell number phenotype of Dis3²/Dis3^G5039

(Snee et al., 2016)

NOT Suppressor of

Statement

Reference

vas^αTub67C.GFP, Scer\GAL4^{VP16.nanos.UTR}, grk^2B6, grk[+] is a non-suppressor of abnormal cell polarity | oogenesis phenotype of Scer\GAL4^{VP16.nanos.UTR}, bru1^UASp.cFa

(Filardo and Ephrussi, 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

grk^2B6 has dorsal appendage phenotype, enhanceable by CSN5[+]/CSN5^ex15

(Doronkin et al., 2002)

grk^2B6 has dorsal appendage phenotype, enhanceable by CSN5^ex35/CSN5[+]

(Doronkin et al., 2002)

grk^2B6 has dorsal appendage phenotype, enhanceable by CSN5[+]/CSN5^ex9

(Doronkin et al., 2002)

grk^2B6 has dorsal appendage phenotype, enhanceable by CSN5^L4032/CSN5[+]

(Doronkin et al., 2002)

NOT Enhanced by

Statement

Reference

grk^2B6 has dorsal appendage phenotype, non-enhanceable by CSN5^ex27/CSN5[+]

(Doronkin et al., 2002)

Suppressed by

Statement

Reference

grk^2B6/grk^2E12 has egg chorion phenotype, suppressible | partially by Dwil\GK18382^+ta

(Niepielko and Yakoby, 2014)

grk^2B6/grk^2E12 has dorsal appendage phenotype, suppressible | partially by Dwil\GK18382^+ta

(Niepielko and Yakoby, 2014)

Enhancer of

Statement

Reference

grk^2B6/grk[+] is an enhancer of dorsal appendage phenotype of Scer\GAL4^{VP16.nanos.UTR}, kek1^UASp.cGa

(Ghiglione et al., 2003)

Suppressor of

Statement

Reference

grk³/grk^2B6 is a suppressor of follicle cell | increased number phenotype of Dis3²/Dis3^G5039

(Snee et al., 2016)

NOT Suppressor of

Statement

Reference

grk³/grk^2B6 is a non-suppressor of dorsal appendage phenotype of cic^fet-U6/cic^fet-T6

(Atkey et al., 2006)

grk³/grk^2B6 is a non-suppressor of egg phenotype of cic^fet-U6/cic^fet-T6

(Atkey et al., 2006)

Other

Statement

Reference

Scer\GAL4^CY2, dpp^UAS.cSa, grk^2B6/grk^HF has egg chorion phenotype

(Shravage et al., 2007)

Scer\GAL4^CY2, dpp^UAS.cSa, grk^2B6/grk^HF has egg phenotype

(Shravage et al., 2007)

Scer\GAL4^CY2, dpp^UAS.cSa, grk^2B6/grk^HF has micropyle phenotype

(Shravage et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

grk³/grk^2B6 suppresses the over proliferation of posterior follicle cells in Dis3^G5039/Dis3² egg chambers.

(Snee et al., 2016)

Eggs derived from females expressing dpp^Scer\UAS.cSa under the control of Scer\GAL4^CY2 in a grk^2B6/grk^HF background have misshaped micropyles, show the loss of the collar that normally separates the operculum from the rest of the chorion and show a reduction in egg diameter.

(Shravage et al., 2007)

Stage 12 grk³/grk^2B6 ; cic^fet-U6/cic^fet-T6 double mutant egg chambers lack dorsal appendages (characteristic of loss of grk), but retain a pronounced collar of appendage material, indicating that loss of grk cannot suppress the cic phenotype.

(Atkey et al., 2006)

Expression of grk::yl^{grk1-275.yl1826-1984} is able to suppress the grk^2B6/grk^HF oogenesis phenotype.

grk::yl^{grk1-275.yl1826-1984}.

(Bokel et al., 2006)

Heterozygosity for grk^2B6 enhances the penetrance of dorsal appendage loss or fusion in eggs laid by Scer\GAL4^{nos.UTR.T:Hsim\VP16}; aret^{Scer\UAS.P\T.cFa} females, from 5% to 90%. This phenotype still shows over 90% penetrance in eggs laid by Scer\GAL4^{nos.UTR.T:Hsim\VP16}; aret^{Scer\UAS.P\T.cFa}; grk^2B6/+; vas^{αTub67C.T:Avic\GFP} flies.

(Filardo and Ephrussi, 2003)

The penetrance and expressivity of the weak ventralization phenotype seen in eggs laid by kek1^{Scer\UAS.P\T.cGa}; Scer\GAL4^{nos.UTR.T:Hsim\VP16} females is strongly enhanced by heterozygosity for grk^2B6.

(Ghiglione et al., 2003)

The fused dorsal appendage phenotype of eggs laid by grk^2B6/+ females is enhanced if the females are also heterozygous for CSN5^L4032, CSN5^ex15, CSN5^ex9 or CSN5^ex35 but is not enhanced if they are also heterozygous for CSN5^ex27.

(Doronkin et al., 2002)

Xenogenetic Interactions

Statement

Reference

The presence of one or two copies of Dwil\GK18382^+ta in grk^2B6/grk^2E12 mutant D. melanogaster flies results in four distinct eggshell phenotypes. Most eggshells, 69% and 78% from one and two copies of Dwil\GK18382^+ta, respectively, are wild-type or wild-type with an enlarger area posterior to the base of the dorsal appendages, similar to wild-type flies expressing Dwil\GK18382^+ta. A dorsal ridge-like morphology is found in 10% and 1% of the eggshells from one and two copies of Dwil\GK18382^+ta, respectively.

In 13% and 18% of the eggshells from in grk^2B6/grk^2E12 mutant D. melanogaster with one and two copies of Dwil\GK18382^+ta, respectively, there is a single or fused dorsal appendage.

In 8% and 3% of the eggshells from in grk^2B6/grk^2E12 mutant D. melanogaster with one and two copies of Dwil\GK18382^+ta, respectively, the eggshells are collapsed and the eggs unmountable.

(Niepielko and Yakoby, 2014)

Complementation and Rescue Data

Rescued by

grk^2B6 is rescued by grk^12xTag:MS2

(Jaramillo et al., 2008)

grk^2B6/grk^2E12 is rescued by grk^ΔC

(Herpers and Rabouille, 2004)

Partially rescued by

grk^2B6 is partially rescued by grk^ΔC

(Queenan et al., 1999)

grk^2B6 is partially rescued by grk^t5

(Queenan et al., 1999)

Not rescued by

grk^2B6/grk^2E12 is not rescued by grk^ΔTC

(Herpers and Rabouille, 2004)

grk^2B6 is not rescued by grk^ΔTC

(Queenan et al., 1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

grk 2B

(Queenan et al., 1999)

grk^2B

(Snee et al., 2016, Becalska and Gavis, 2010, Jaramillo et al., 2008, Cui et al., 2005, Ghiglione et al., 2003, Dollar et al., 2002, Doronkin et al., 2002)

grk^2Bb

(Niepielko and Yakoby, 2014)

grk^2b6

(Duchek and Rorth, 2001)

Name Synonyms

Secondary FlyBase IDs

FBal0082909

References (56)

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