FlyBase Allele Report: Dmel\Pp1-87B[87Bg-3]

General Information

Symbol

Dmel\Pp1-87B^87Bg-3

Species

D. melanogaster

Name

FlyBase ID

FBal0013938

Feature type

allele

Associated gene

Dmel\Pp1-87B

Associated Insertion(s)

Carried in Construct

Also Known As

PP1α87B^87Bg-3, PP1α87B^e211, ck19^e211

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Axton et al., 1990)

Mutagen

ethyl methanesulfonate

(Baksa et al., 1993)

Progenitor genotype

Cytology

Polytene chromosomes normal.

(Axton et al., 1990)

Description

There may be a DNA rearrangement at the 5' end of the Pp1-87B^87Bg-3 gene, however no lesion was detected cytologically.

(Axton et al., 1990)

Sequence 165bp and more 5' upstream of the ATG initiation site diverges from the wild-type sequence due to a deletion of DNA. This removes the TATA box.

(Dombradi et al., 1990)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:12,425,853..12,425,853 [-]

Comment:

Deletion with a breakpoint 165bp upstream of the ATG initiation site. This removes the TATA box and other upstream sequences. Extent of deletion unknown.

(Dombradi et al., 1990)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval optic lobe (with Pp1-87B^87Bg-6)

(Babu et al., 2005)

eye | somatic clone

(Jiang et al., 2011)

eye disc | somatic clone

(Jiang et al., 2011)

eye photoreceptor cell | somatic clone

(Jiang et al., 2011)

ganglion | third instar larval stage

(Axton et al., 1990)

ganglion mother cell

(Baksa et al., 1993)

imaginal disc

(Axton et al., 1990)

mitosis & nuclear chromosome

(Bennett et al., 2003)

mitotic spindle | third instar larval stage

(Axton et al., 1990)

neuroblast

(Baksa et al., 1993)

photoreceptor cell R1 & axon (with Pp1-87B^87Bg-6)

(Babu et al., 2005)

photoreceptor cell R2 & axon (with Pp1-87B^87Bg-6)

(Babu et al., 2005)

photoreceptor cell R3 & axon (with Pp1-87B^87Bg-6)

(Babu et al., 2005)

photoreceptor cell R4 & axon (with Pp1-87B^87Bg-6)

(Babu et al., 2005)

photoreceptor cell R5 & axon (with Pp1-87B^87Bg-6)

(Babu et al., 2005)

Detailed Description

Statement

Reference

Pp1-87B^87Bg-3 Pp1α-96A² double heterozygous embryos exhibit damped (and spatially more confined) oscillations in the Cdk1/PP1 activity ratio, reduced cortical contractions and cytoplasmic flows, non-uniform positioning, tissue-wide asynchronies, and anaphase delays when compared to controls.

(Deneke et al., 2019)

Eye morphogenesis is disrupted and eye size is reduced in animals containing eyes that are almost completely homozygous for Pp1-87B^87Bg-3. In addition, mutant photoreceptor cells, cone cells and undifferentiated cells are mislocalised in the optic stalk of these animals.

(Jiang et al., 2011)

Pp1-87B¹/Pp1-87B^87Bg-3 animals die during pupariation, and until pupariation, do not lag behind in development compared to their control siblings.

(Kirchner et al., 2007)

Pp1-87B^87Bg-3/Pp1-87B^87Bg-6 larvae show axon clumping at the lamina and laminal breaks with ~50% of R2-R5 axons being mistargeted to the medulla.

(Babu et al., 2005)

Squash preparations of Pp1-87B^87Bg-3/Df(3R)kar-H10 third larval instar brains contain fewer cells per microscope field than normal and a significantly higher proportion of cells in mitosis than in wild type. Overcondensed chromosomes are seen.

(Bennett et al., 2003)

Weak suppressor levels of position effect vareigation and a high frequency of aberrant mitosis in neuroblasts or ganglion mother cells. Metaphase chromosomes and anaphase chromatids are over condensed.

(Baksa et al., 1993)

Larvae die either just before or just after pupariation. Third instar larval imaginal discs are underdeveloped, indicating failure of diploid cell proliferation. Ganglia are smaller than those of wild-type larvae, with significantly fewer cells per microscope field in squash preparations, suggesting cell division is impaired in the ganglia. The proportion of cells in mitosis in the ganglia is significantly higher than in wild type. This is due to a large number of mitotic cells showing abnormalities, such as overcondensed or extra chromosomes. These cells are probably delayed in progress through mitosis. Spindle organisation is also defective, showing unusually little space between almost parallel bundles of spindle microtubules at metaphase.

(Axton et al., 1990)

L2/L3 larval lethal

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pp1-87B^87Bg-3/Pp1-87B¹ has lethal phenotype, suppressible by Scer\GAL4^arm.PS/Pp1α-96A^UAS.Tag:HA

(Kirchner et al., 2007)

Pp1-87B^87Bg-3/Pp1-87B¹ has lethal phenotype, suppressible by flw^UAS.Tag:HA/Scer\GAL4^arm.PS

(Kirchner et al., 2007)

NOT suppressed by

Statement

Reference

Pp1α-96A², Pp1-87B^87Bg-3/Pp1-87B¹ has lethal phenotype, non-suppressible by flw^UAS.Tag:HA/Scer\GAL4^arm.PS

(Kirchner et al., 2007)

Suppressor of

Statement

Reference

Pp1-87B^87Bg-3/Pp1-87B[+] is a suppressor of viable phenotype of Nop17l^{UAS.Tag:polyHis,Tag:MYC}, Scer\GAL4^arm.PS

(Bennett et al., 2006)

NOT Suppressor of

Statement

Reference

Scer\GAL4^arm.PS, Pp1-87B^87Bg-3, Pp1-87B^UAS.Tag:HA, Pp1-87B[+] is a non-suppressor of viable phenotype of Nop17l^{UAS.Tag:polyHis,Tag:MYC}, Scer\GAL4^arm.PS

(Bennett et al., 2006)

Other

Statement

Reference

Pp1α-96A², Pp1-87B^87Bg-3 has abnormal cell cycle | embryonic stage phenotype

(Deneke et al., 2019)

Pp1-87B^87Bg-3/Pp1-87B[+], flw¹ has viable phenotype

(Kirchner et al., 2007)

Pp1α-96A², Pp1-87B^87Bg-3/Pp1-87B[+] has viable phenotype

(Kirchner et al., 2007)

Phenotype Manifest In

Suppressed by

Statement

Reference

Pp1α-96A², Pp1-87B^87Bg-3 has embryo phenotype, suppressible | partially by grp²⁰⁹/grp^Z5170, lok³⁰/lok³⁰

(Deneke et al., 2019)

Enhancer of

Statement

Reference

Pp1-87B^87Bg-3/Pp1-87B[+] is an enhancer of wing phenotype of Nop17l^{UAS.Tag:polyHis,Tag:MYC}, Scer\GAL4^Bx-MS1096

(Bennett et al., 2006)

Pp1-87B^87Bg-3/Pp1-87B[+] is an enhancer of posterior wing margin phenotype of Nop17l^{UAS.Tag:polyHis,Tag:MYC}, Scer\GAL4^Bx-MS1096

(Bennett et al., 2006)

Pp1-87B^87Bg-3, Pp1-87B[+], Df(3R)crb87-5, + is an enhancer of wing blade posterior compartment phenotype of Scer\GAL4^vg.int2.1, put^UAS.cMa

(Bennett and Alphey, 2002)

Suppressor of

Statement

Reference

Pp1-87B^87Bg-3 is a suppressor of adult prothoracic segment phenotype of E(z)^Trm

(Bajusz et al., 2001)

Pp1-87B^87Bg-3 is a suppressor of adult abdominal segment 4 | ectopic phenotype of E(z)^Trm

(Bajusz et al., 2001)

Pp1-87B^87Bg-3 is a suppressor of adult abdominal segment 5 phenotype of E(z)^Trm

(Bajusz et al., 2001)

Pp1-87B^87Bg-3 is a suppressor of adult mesothoracic segment | ectopic phenotype of E(z)^Trm

(Bajusz et al., 2001)

Pp1-87B^87Bg-3 is a suppressor of adult metathoracic segment phenotype of E(z)^Trm

(Bajusz et al., 2001)

Other

Statement

Reference

Pp1α-96A², Pp1-87B^87Bg-3 has embryo phenotype

(Deneke et al., 2019)

Df(3R)crb87-5/+, Pp1-87B^87Bg-3/Pp1-87B[+], Scer\GAL4^vg.int2.1, put^UAS.cMa has wing margin bristle phenotype

(Bennett and Alphey, 2002)

Df(3R)crb87-5/+, Pp1-87B^87Bg-3/Pp1-87B[+], Scer\GAL4^vg.int2.1, put^UAS.cMa has anterior wing margin phenotype

(Bennett and Alphey, 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Pp1-87B^87Bg-3 does not show any discernible mutant phenotype with puc^A251.1F3.

(Kirchner et al., 2007)

Pp1-87B¹/Pp1-87B^87Bg-3 Pp1α-96A²/Pp1α-96A² double mutant animals die slightly earlier than Pp1-87B¹/Pp1-87B^87Bg-3 single mutants and pupariate 1-2 days later than their siblings.

(Kirchner et al., 2007)

Expression of Nop17l^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} under the control of Scer\GAL4^Bx-MS1096 in Pp1-87B^87Bg-3/+ wings results in severely crumpled and reduced wings.

Expression of Nop17l^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} under the control of Scer\GAL4^arm.PS is synthetic lethal in a heterozygous Pp1-87B^87Bg-3 mutant background.

The synthetic lethality resulting from the overexpression of Nop17l^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} under the control of Scer\GAL4^arm.PS in a heterozygous Pp1-87B^87Bg-3 mutant background can be rescued by the co-expression of Pp1-87B^{Scer\UAS.T:Ivir\HA1}.

(Bennett et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Pp1-87B^87Bg-3/Pp1-87B¹ is rescued by Pp1-87B^UAS.Tag:HA/Scer\GAL4^arm.PS

(Kirchner et al., 2007)

Pp1-87B^87Bg-3 is rescued by Pp1-87B^+t5.8

(Baksa et al., 1993)

Comments

The lethality of Pp1-87B^87Bg-3 is rescued by Pp1-87B^+t5.8.

(Baksa et al., 1993)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

23696

w^*; Pp1-87B^87Bg-3/TM6B, Tb¹

23699

w^*; Pp1-87B^87Bg-3 e¹ Pp1α-96A²/TM6B, Tb¹

Notes on Origin

Discoverer

Comments

Not suppressed by zip alleles.

(Vereshchagina et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

PP1α-87B^87Bg3

(Jiang et al., 2011)

PP1α87B^87Bg-3

(Moura et al., 2025, Kirchner et al., 2007, Bennett et al., 2006, Vereshchagina et al., 2004)

PP1α87B^e211

(Babu et al., 2005, Bennett et al., 2003)

Pp1-87B^87Bg-3

(Deneke et al., 2019, Kirchner et al., 2007)

Pp1-87B^Bg3

(Roubinet et al., 2017)

Pp1-87B^e211

(Bennett and Alphey, 2002)

ck19^e211

(Axton et al., 1990, Dombradi et al., 1990)

e211

(Baksa et al., 1993)

e²¹¹

(Gausz et al., 1981)

l(3)87Bg³

pp1-87B^87Bg-3

(Moreira et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (18)

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