FlyBase Allele Report: Dmel\Egfr[f8]

General Information

Symbol

Dmel\Egfr^f8

Species

D. melanogaster

Name

FlyBase ID

FBal0003536

Feature type

allele

Associated gene

Dmel\Egfr

Associated Insertion(s)

Carried in Construct

Also Known As

flb^2X51, top^2X51

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Clifford and Schupbach, 1989, Schejter and Shilo, 1989)

Mutagen

ethyl methanesulfonate

(Clifford and Schupbach, 1994, Clifford and Schupbach, 1989, Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Nucleotide substitution: T3989GA. +1 frameshift mutation.

(Clifford and Schupbach, 1994)

Nucleotide substitution: T3480G. Amino acid replacement: D1160E. There is also a 1bp insertion (A) after nucleotide 3482, which results in a frame shift at amino acid 1161, and leads to premature termination after a further 12 amino acids. This removes the entire C-terminal tail, while leaving the kinase domain intact.

(Raz et al., 1991)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:21,558,431..21,558,431 [+]

Nucleotide change:

T21558431A

Reported nucleotide change:

T3989GA

Amino acid change:

D1161E | Egfr-PA; D1210E | Egfr-PB

Comment:

+1 frameshift

(Clifford and Schupbach, 1994, Raz et al., 1991)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage | recessive

(Clifford and Schupbach, 1994, Clifford and Schupbach, 1989, Schejter and Shilo, 1989)

some die during embryonic stage | recessive

(Clifford and Schupbach, 1994, Clifford and Schupbach, 1989, Schejter and Shilo, 1989)

Phenotype Manifest In

embryonic head

(Raz et al., 1991)

ventral denticle belt

(Raz et al., 1991)

Detailed Description

Statement

Reference

Transheterozygous females with Egfr^t1 display egg chambers with gaps in the follicular epithelium that uncover the nurse cells. Eggs derived from these females display weak or intermediate ventralised phenotypes.

(Goode et al., 1996)

Homozygotes have a severe embryonic phenotype; denticles are missing or severely reduced, and there are head and germ band retraction defects. Complements Egfr^f6 and Egfr^f4; milder embryonic phenotypes (in both the cuticle and CNS) are seen in combination with either of these alleles. Shows a rough eye phenotype in combination with Egfr^E1.

(Raz et al., 1991)

Homozygotes and hemizygotes display a severe 'flb' phenotype. Adults preferentially retain eye and bristle Egfr activity. Embryos produced from heteroallelic combination with Egfr^t1 have a severe ventralised phenotype, reduction in size of their dorsal appendage.

(Clifford and Schupbach, 1989)

severe allele

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

Egfr^f8/Egfr[+] is a suppressor | partially of egg chorion phenotype of Egfr::kek1^{KEΔCG.UAS.GFP}, Scer\GAL4^CY2

(Alvarado et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

The dorsalised chorion phenotype caused by expression of Egfr::kek1^{KEΔCG.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^CY2 is partially suppressed if the flies are also heterozygous for Egfr^f8.

(Alvarado et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: Embryonic lethal.

Comments

Mutation of Egfr that affects the gene function required for imaginal disc derivatives, a class IV lesion.

(Clifford and Schupbach, 1989)

Class IIB allele. Class II alleles fully or partially complement the developmental defects of Egfr^t1 and Egfr^top-CA. Substantially complements the postembryonic lethality of Egfr^top-EC20. Several combinations of Egfr alleles involving Egfr^top-101, Egfr^f4, Egfr^f8 and Egfr^f6 demonstrate interallelic complementation.

(Clifford and Schupbach, 1994)

External Crossreferences and Linkouts ( 2 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

X78919

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

CAA55522

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Egfr^f8

egfr^2X51

(Alvarado et al., 2004)

flb

flb^2X51

(Schweitzer et al., 1995, Raz and Shilo, 1993, Raz et al., 1991, Schejter and Shilo, 1989)

flb^IIX

(Tearle and Nusslein-Volhard, 1987)

top^2X51

(Goode et al., 1996, Clifford and Schupbach, 1994, Clifford and Schupbach, 1989)

Name Synonyms

faint little ball

Secondary FlyBase IDs

References (10)

Report Sections

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