FlyBase Allele Report: Dmel\cuff[WM25]

General Information

Symbol

Dmel\cuff^WM25

Species

D. melanogaster

Name

FlyBase ID

FBal0002144

Feature type

allele

Associated gene

Dmel\cuff

Associated Insertion(s)

Carried in Construct

Also Known As

cuff^WM

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Schupbach and Wieschaus, 1991)

Progenitor genotype

Cytology

Description

Amino acid replacement: M1K.

(Chen et al., 2007)

Nucleotide substitution: T2A.

(Chen et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:11,886,112..11,886,112 [+]

Nucleotide change:

T11886112A

Reported nucleotide change:

T?A

Amino acid change:

M1K | cuff-PA

Reported amino acid change:

M1K

(Chen et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile

(Chen et al., 2007, Schupbach and Wieschaus, 1991)

Phenotype Manifest In

egg chorion

(Chen et al., 2007)

egg chorion (with cuff^KG05951)

(Chen et al., 2007)

egg chorion (with cuff^QQ37)

(Chen et al., 2007)

female fusome (with cuff^KG05951)

(Chen et al., 2007)

female germline cyst (with cuff^KG05951)

(Chen et al., 2007)

germarium (with cuff^KG05951)

(Chen et al., 2007)

oocyte nucleus (with cuff^KG05951)

(Chen et al., 2007)

Detailed Description

Statement

Reference

Eggs laid by cuff^WM25, cuff^WM25/cuff^KG05951 and cuff^WM25/cuff^QQ37 females show a variable ventralised phenotype.

The oocyte nucleus has defective morphology in 10-20% of stage 9 egg chambers in cuff^WM25/cuff^KG05951 females, often the DNA seems to localise to the periphery of the nucleus.

In newly eclosed cuff^WM25/cuff^KG05951 females, highly branched fusomes are observed in regions 1 and 2 of the germaria (as occurs in wild-type females). However, as the mutant females age, there is a sharp increase in the number of germaria without cysts that contain highly branched fusomes; 1 week after eclosion, 54% of the germaria do not have highly branched fusomes and 2 weeks after eclosion, 83% of the germaria do not have highly branched fusomes in the mutant females. The mutant cysts in older female appear to arrest in early stages in the germaria containing spectrosome-like structures. In addition, the overall morphology of germline cysts in region 2b and/or region 3 of the germaria is also disrupted in cuff^WM25/cuff^KG05951 females; 1 week after eclosion, 72% of the germaria do not have cysts of normal morphology and 2 weeks after eclosion, 84% of the germaria do not have cysts of normal morphology.

(Chen et al., 2007)

Absence of egg chambers or degeneration of early egg chambers.

(Schupbach and Wieschaus, 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

cuff^WM25/cuff[+] is an enhancer of abnormal meiotic cell cycle phenotype of RanGAP^Sd

(Gell and Reenan, 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

cuff^WM25/cuff^QQ37 has egg chorion phenotype, suppressible | partially by lok^unspecified

(Chen et al., 2007)

cuff^WM25 has egg chorion phenotype, suppressible by lok^unspecified

(Chen et al., 2007)

cuff^WM25 has female fusome phenotype, suppressible by lok^unspecified

(Chen et al., 2007)

cuff^WM25 has female germline cyst phenotype, suppressible by lok^unspecified

(Chen et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The segregation distortion seen in males carrying either the SD-5star or SD-LosArenos Dp(2;2)RanGAP^SD chromosome variant (in a sensitive Rsp background) is enhanced (the k[[c]] value, or proportion of Dp(2;2)RanGAP^SD-bearing progeny as the fraction of total progeny (corrected for viability), is significantly increased) if the males are also carrying a single copy of cuff^WM25.

(Gell and Reenan, 2013)

The ventralised phenotype seen in eggs derived from cuff^WM25 and cuff^WM25/cuff^QQ37 females is partially suppressed by lok^unspecified. The defects in germline cyst development seen in cuff^WM25 females are partially suppressed by lok^unspecified; many highly branched fusomes are seen in the double mutant germaria.

(Chen et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

cuff³

cuff^WM25

(Chen et al., 2021, Parhad et al., 2020, Pritykin et al., 2017, Hur et al., 2016, Kibanov et al., 2011, Pane et al., 2011, Chen et al., 2007, Chen et al., 2007)

cuff^WM

(Parhad et al., 2020, Wylie et al., 2014, Gell and Reenan, 2013)

cuff^wm25

(Chen et al., 2016, Zhang et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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