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ENZYME

ENZYME entry: EC 1.14.13.39

PURL: https://purl.expasy.org/enzyme/EC/1.14.13.39
Accepted Name
nitric-oxide synthase (NADPH)
Alternative Name(s)
endothelium-derived relaxation factor-forming enzyme
endothelium-derived relaxing factor synthase
NADPH-diaphorase
nitric-oxide synthetase
NO synthase
Reaction catalysed
2 L-arginine + 3 NADPH + 4 O2 + H(+) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H2O
Comment(s)
  • The enzyme consists of linked oxygenase and reductase domains.
  • The eukaryotic enzyme binds FAD, FMN, heme (iron protoporphyrin IX) and tetrahydrobiopterin, and its two domains are linked via a regulatory calmodulin-binding domain.
  • Upon calcium-induced calmodulin binding, the reductase and oxygenase domains form a complex, allowing electrons to flow from NADPH via FAD and FMN to the active center.
  • The reductase domain of the enzyme from the bacterium Sorangium cellulosum utilizes a [2Fe-2S] cluster to transfer the electrons from NADPH to the active center. cf. EC 1.14.14.47.
Cross-references
BRENDA1.14.13.39
EC2PDB1.14.13.39
ExplorEnz1.14.13.39
KEGG Ligand Database for Enzyme Nomenclature1.14.13.39
IUBMB Enzyme Nomenclature1.14.13.39
MEDLINEFind literature relating to 1.14.13.39
MetaCyc1.14.13.39
Rhea expert-curated reactions1.14.13.39
UniProtKB/Swiss-Prot
Q66GP9, NOA1_ARATHP29475, NOS1_HUMANQ9Z0J4, NOS1_MOUSE
O19132, NOS1_RABITP29476, NOS1_RATQ29498, NOS1_SHEEP
Q27995, NOS2_BOVINO62699, NOS2_CANLFQ28314, NOS2_CAPHI
Q92037, NOS2_CARAUO54705, NOS2_CAVPOQ90703, NOS2_CHICK
P35228, NOS2_HUMANO46660, NOS2_MACMUP29477, NOS2_MOUSE
Q92091, NOS2_ONCMYP79290, NOS2_PIGO19114, NOS2_RABIT
Q06518, NOS2_RATP29473, NOS3_BOVINQ9TUX8, NOS3_CANLF
P97270, NOS3_CAVPOP29474, NOS3_HUMANP70313, NOS3_MOUSE
Q28969, NOS3_PIGQ62600, NOS3_RATP79209, NOS3_SHEEP
B1B557, NOSL_BOMMOO61608, NOS_ANOSTQ8T8C0, NOS_BOMMO
Q27571, NOS_DROMEO61309, NOS_LYMSTQ6YPG5, NOS_ORYSJ
Q26240, NOS_RHOPRQ9I9M2, NOS_SQUAC

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